TY - JOUR
T1 - Effects of Clostridium Difficile toxins A and B on cytoskeleton organization in HEp-2 cells
T2 - A comparative morphological study
AU - Fiorentini, C.
AU - Arancia, G.
AU - Paradisi, S.
AU - Donelli, G.
AU - Giuliano, M.
AU - Piemonte, F.
AU - Mastrantonio, P.
PY - 1989
Y1 - 1989
N2 - C. Fiorentini, G. Arancia, S. Paradisi, G. Donelli, M. Giuliano, F. Piemonte and P. Mastrantonio. Effects of Clostridium difficile toxins A and B on cytoskeleton organization in HEp-2 cells: a comparative morphological study. Toxicon27, 1209-1218, 1989.-A comparative study on the effects of toxin A and toxin B from Clostridium difficile on HEp-2 cells was carried out. Both toxins caused cell retraction and rounding and seemed to exert their effect on cell morphology via a rearrangement of actin and α-actinin microfilaments. Such a rearrangement occurred at an early stage, when no change in microtubular and cytokeratin systems was detectable. Nevertheless, several structural modifications accompanying the cytopathological process induced by toxins A and B appeared to be quite different. In particular, toxin B-treated cells showed an arborized phenotype as a result of cell retraction and rounding, whereas toxin A caused cell rounding without arborization. Moreover, nuclear polarization following disorganization of the microfilament system was only observed in toxin A-treated cells. The structural features distinguishing intoxication processes induced by the two toxins probably reflect a different mechanism of action and suggest the presence of a distinct subcellular component as a primary target for each toxin.
AB - C. Fiorentini, G. Arancia, S. Paradisi, G. Donelli, M. Giuliano, F. Piemonte and P. Mastrantonio. Effects of Clostridium difficile toxins A and B on cytoskeleton organization in HEp-2 cells: a comparative morphological study. Toxicon27, 1209-1218, 1989.-A comparative study on the effects of toxin A and toxin B from Clostridium difficile on HEp-2 cells was carried out. Both toxins caused cell retraction and rounding and seemed to exert their effect on cell morphology via a rearrangement of actin and α-actinin microfilaments. Such a rearrangement occurred at an early stage, when no change in microtubular and cytokeratin systems was detectable. Nevertheless, several structural modifications accompanying the cytopathological process induced by toxins A and B appeared to be quite different. In particular, toxin B-treated cells showed an arborized phenotype as a result of cell retraction and rounding, whereas toxin A caused cell rounding without arborization. Moreover, nuclear polarization following disorganization of the microfilament system was only observed in toxin A-treated cells. The structural features distinguishing intoxication processes induced by the two toxins probably reflect a different mechanism of action and suggest the presence of a distinct subcellular component as a primary target for each toxin.
UR - http://www.scopus.com/inward/record.url?scp=0024472054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024472054&partnerID=8YFLogxK
U2 - 10.1016/0041-0101(89)90029-9
DO - 10.1016/0041-0101(89)90029-9
M3 - Article
C2 - 2515619
AN - SCOPUS:0024472054
VL - 27
SP - 1209
EP - 1218
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 11
ER -