Effects of corticotrophin releasing factor, muscarine and somatostatin on rubidium and potassium efflux from mouse AtT-20 pituitary cells

A. Luini, D. A. Brown

Research output: Contribution to journalArticle

Abstract

Effects of secretagogues and anti-secretagogues of ACTH secretion on K+ permeability in the clonal pituitary cell line AtT-20 were measured by recording 86Rb or 42K efflux. Efflux was accelerated by the secretagogues K+, corticotrophin, forskolin, isoprenaline, and the Ca-ionophore A23187. Efflux was reduced by the inhibitors somatostatin, muscarine, and oxotremorine, or by removing external Ca. Efflux was also reduced by the K+ -channel blocking compound d-tubocurarine but not by tetraethylammonium. Muscarine, oxotremorine, somatostatin, and 0 Ca2+ also reduced intracellular Ca2+ measured by quin-2 fluorescence. It is suggested that most of the resting 86Rb or 42K efflux measured under these conditions occurs via tubocurarine-sensitive Ca2+-dependent K+-channels and that changes in efflux rate produced by secretagogues or anti-secretagogues are secondary to changes in intracellular Ca2+.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalEuropean Journal of Neuroscience
Volume2
Issue number2
DOIs
Publication statusPublished - 1990

Keywords

  • Corticotrophin releasing factor
  • Muscarine
  • Pituitary cells
  • Rubidium flux
  • Somatostatin

ASJC Scopus subject areas

  • Neuroscience(all)

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