Objective: This study was designed to evaluate the combined effect of CO2 and various cystalloid solutions on human mesothelial cells in an in vitro system. We focused on the apposition ability as a factor contributing to the initial adhesion step leading to adhesion formation. Methods: Samples of human peritoneum were obtained from 11 patients. Adhesion ability was measured using a binding assay between a mesothelial cell monolayer pretreated with a CO2 flow and 51Cr-labeled mesothelial cells. Radioactivity of 51Cr release was used as an indicator of adherent mesothelial cells. Results: The adherence of mesothelial cells to mesothelial monolayers was significantly reduced using saline with NaHCO3 when compared to saline solution alone. No significant differences were observed between saline with NaHCO3 and lactated Ringer's solution. Conclusion: Saline with NaHCO3, being a better buffer than normal saline, may be proposed as an alternative solution to prevent peritoneal cell apposition and adhesion.
|Number of pages||5|
|Journal||Italian Journal of Gynaecology and Obstetrics|
|Publication status||Published - 1997|
- Crystalloid solution
ASJC Scopus subject areas
- Obstetrics and Gynaecology