Effects of crystalloid solutions used in adhesion prevention on human mesothelial cells

Objective: This study was designed to evaluate the combined effect of CO₂ and various cystalloid solutions on human mesothelial cells in an in vitro system. We focused on the apposition ability as a factor contributing to the initial adhesion step leading to adhesion formation. Methods: Samples of human peritoneum were obtained from 11 patients. Adhesion ability was measured using a binding assay between a mesothelial cell monolayer pretreated with a CO₂ flow and ⁵¹Cr-labeled mesothelial cells. Radioactivity of ⁵¹Cr release was used as an indicator of adherent mesothelial cells. Results: The adherence of mesothelial cells to mesothelial monolayers was significantly reduced using saline with NaHCO₃ when compared to saline solution alone. No significant differences were observed between saline with NaHCO₃ and lactated Ringer's solution. Conclusion: Saline with NaHCO₃, being a better buffer than normal saline, may be proposed as an alternative solution to prevent peritoneal cell apposition and adhesion.