Effects of cyclic adenosine-monophosphate on growth and PSA secretion of human prostate cancer cell line.

V. Macchia, A. Di Carlo, C. De Luca, A. Mariano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Prolonged increase of cyclic adenosine-monophosphate (cAMP) level in the culture medium of a well differentiated human prostatic cancer cell (LNCaP) inhibits cellular growth and stimulates PSA secretion. The differentiation of the cells tested was documented by their responsiveness to androgens and the ability to synthesize cellular markers of differentiation (PSA). The raise in cAMP level was produced by dibutyryl cyclic AMP (DBcAMP) or by agents acting at distinct levels in the pathway of cAMP generation (forskolin) or degradation (IBMX). Each of these three agents in a range of concentrations between 10-4-10-6 M had an inhibitory effect on the growth which is dose and time-dependent. The inhibition was reversible as demonstrated by complete restoration of cell growth soon after the withdrawal of the substances from the culture medium. When cAMP levels in culture medium was raised, an increase in PSA content was observed. However, the effects of cAMP on PSA content was not due to increase in PSA synthesis, since simultaneous measurement of secreted and cellular PSA indicated that the principal effect of the cyclic nucleotide was to enhance the secretion of stored PSA. Furthermore the inhibition of cellular growth by cAMP suggests new approaches in prostatic carcinoma therapy.

Original languageEnglish
Pages (from-to)1071-1076
Number of pages6
JournalInternational Journal of Oncology
Volume18
Issue number5
Publication statusPublished - May 2001

Fingerprint

Cyclic AMP
Prostatic Neoplasms
Cell Line
Growth
Culture Media
1-Methyl-3-isobutylxanthine
Bucladesine
Cyclic Nucleotides
Differentiation Antigens
Colforsin
Androgens
Cell Differentiation
Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects of cyclic adenosine-monophosphate on growth and PSA secretion of human prostate cancer cell line. / Macchia, V.; Di Carlo, A.; De Luca, C.; Mariano, A.

In: International Journal of Oncology, Vol. 18, No. 5, 05.2001, p. 1071-1076.

Research output: Contribution to journalArticle

Macchia, V. ; Di Carlo, A. ; De Luca, C. ; Mariano, A. / Effects of cyclic adenosine-monophosphate on growth and PSA secretion of human prostate cancer cell line. In: International Journal of Oncology. 2001 ; Vol. 18, No. 5. pp. 1071-1076.
@article{cafb371b1ad44a1285c3cd341648c591,
title = "Effects of cyclic adenosine-monophosphate on growth and PSA secretion of human prostate cancer cell line.",
abstract = "Prolonged increase of cyclic adenosine-monophosphate (cAMP) level in the culture medium of a well differentiated human prostatic cancer cell (LNCaP) inhibits cellular growth and stimulates PSA secretion. The differentiation of the cells tested was documented by their responsiveness to androgens and the ability to synthesize cellular markers of differentiation (PSA). The raise in cAMP level was produced by dibutyryl cyclic AMP (DBcAMP) or by agents acting at distinct levels in the pathway of cAMP generation (forskolin) or degradation (IBMX). Each of these three agents in a range of concentrations between 10-4-10-6 M had an inhibitory effect on the growth which is dose and time-dependent. The inhibition was reversible as demonstrated by complete restoration of cell growth soon after the withdrawal of the substances from the culture medium. When cAMP levels in culture medium was raised, an increase in PSA content was observed. However, the effects of cAMP on PSA content was not due to increase in PSA synthesis, since simultaneous measurement of secreted and cellular PSA indicated that the principal effect of the cyclic nucleotide was to enhance the secretion of stored PSA. Furthermore the inhibition of cellular growth by cAMP suggests new approaches in prostatic carcinoma therapy.",
author = "V. Macchia and {Di Carlo}, A. and {De Luca}, C. and A. Mariano",
year = "2001",
month = "5",
language = "English",
volume = "18",
pages = "1071--1076",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Effects of cyclic adenosine-monophosphate on growth and PSA secretion of human prostate cancer cell line.

AU - Macchia, V.

AU - Di Carlo, A.

AU - De Luca, C.

AU - Mariano, A.

PY - 2001/5

Y1 - 2001/5

N2 - Prolonged increase of cyclic adenosine-monophosphate (cAMP) level in the culture medium of a well differentiated human prostatic cancer cell (LNCaP) inhibits cellular growth and stimulates PSA secretion. The differentiation of the cells tested was documented by their responsiveness to androgens and the ability to synthesize cellular markers of differentiation (PSA). The raise in cAMP level was produced by dibutyryl cyclic AMP (DBcAMP) or by agents acting at distinct levels in the pathway of cAMP generation (forskolin) or degradation (IBMX). Each of these three agents in a range of concentrations between 10-4-10-6 M had an inhibitory effect on the growth which is dose and time-dependent. The inhibition was reversible as demonstrated by complete restoration of cell growth soon after the withdrawal of the substances from the culture medium. When cAMP levels in culture medium was raised, an increase in PSA content was observed. However, the effects of cAMP on PSA content was not due to increase in PSA synthesis, since simultaneous measurement of secreted and cellular PSA indicated that the principal effect of the cyclic nucleotide was to enhance the secretion of stored PSA. Furthermore the inhibition of cellular growth by cAMP suggests new approaches in prostatic carcinoma therapy.

AB - Prolonged increase of cyclic adenosine-monophosphate (cAMP) level in the culture medium of a well differentiated human prostatic cancer cell (LNCaP) inhibits cellular growth and stimulates PSA secretion. The differentiation of the cells tested was documented by their responsiveness to androgens and the ability to synthesize cellular markers of differentiation (PSA). The raise in cAMP level was produced by dibutyryl cyclic AMP (DBcAMP) or by agents acting at distinct levels in the pathway of cAMP generation (forskolin) or degradation (IBMX). Each of these three agents in a range of concentrations between 10-4-10-6 M had an inhibitory effect on the growth which is dose and time-dependent. The inhibition was reversible as demonstrated by complete restoration of cell growth soon after the withdrawal of the substances from the culture medium. When cAMP levels in culture medium was raised, an increase in PSA content was observed. However, the effects of cAMP on PSA content was not due to increase in PSA synthesis, since simultaneous measurement of secreted and cellular PSA indicated that the principal effect of the cyclic nucleotide was to enhance the secretion of stored PSA. Furthermore the inhibition of cellular growth by cAMP suggests new approaches in prostatic carcinoma therapy.

UR - http://www.scopus.com/inward/record.url?scp=0035344223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035344223&partnerID=8YFLogxK

M3 - Article

C2 - 11295058

AN - SCOPUS:0035344223

VL - 18

SP - 1071

EP - 1076

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 5

ER -