TY - JOUR
T1 - Effects of cyclosporin a on in vitro lymphocyte response to autologous or allogeneic stimulation
T2 - Influence of HLA phenotypes
AU - Scorza, R.
AU - Vanoli, M.
AU - Coppola, C.
AU - Cigognini, A.
AU - Fabio, G.
AU - Zanussi, C.
PY - 1988
Y1 - 1988
N2 - In this study we investigated whether the interindividual variability of lymphocyte sensitivity to cyclosporin A (CsA) could be controlled by the HLA region. The models used were the in vitro primary and secondary autologous (AMLR) and allogneic mixed lymphocyte (MLR) cultures of cells from 32 healthy subjects from our HLA reference panel. Our results show that CsA inhibited primary allogeneic MLR to a much greater extent than primary AMLR (-81 ± 2% vs -38 ± 8%, P <0.001). The same pattern was observed when cells harvested from CsA-treated primary cultures were rechallenged in secondary cultures with the original sensitizing stimulator cells (-40 ± 6% vs -17 ± 9%, P <0.05). No differences were observed in primary autologous and allogeneic cultures among responders of different HLA phenotypes. In contrast, the secondary responses did vary according to the HLA types: in secondary AMLR, CsA-priming did not lower, or even enhance, the proliferative responses of DR5+ and/or DR2+ lymphocytes (+7 ± 13%), whereas it significantly lowered the responses of DR2-5- cells (-46 ± 8%). In secondary MLR, lymphocytes proliferation was lowered by CsA-priming in all but DRW11(5)+ subjects (-45 ± 7% vs +2 ± 23%, P <0.05). It is concluded that the individual HLA phenotype influences the pattern of lymphocyte sensitivity to CsA.
AB - In this study we investigated whether the interindividual variability of lymphocyte sensitivity to cyclosporin A (CsA) could be controlled by the HLA region. The models used were the in vitro primary and secondary autologous (AMLR) and allogneic mixed lymphocyte (MLR) cultures of cells from 32 healthy subjects from our HLA reference panel. Our results show that CsA inhibited primary allogeneic MLR to a much greater extent than primary AMLR (-81 ± 2% vs -38 ± 8%, P <0.001). The same pattern was observed when cells harvested from CsA-treated primary cultures were rechallenged in secondary cultures with the original sensitizing stimulator cells (-40 ± 6% vs -17 ± 9%, P <0.05). No differences were observed in primary autologous and allogeneic cultures among responders of different HLA phenotypes. In contrast, the secondary responses did vary according to the HLA types: in secondary AMLR, CsA-priming did not lower, or even enhance, the proliferative responses of DR5+ and/or DR2+ lymphocytes (+7 ± 13%), whereas it significantly lowered the responses of DR2-5- cells (-46 ± 8%). In secondary MLR, lymphocytes proliferation was lowered by CsA-priming in all but DRW11(5)+ subjects (-45 ± 7% vs +2 ± 23%, P <0.05). It is concluded that the individual HLA phenotype influences the pattern of lymphocyte sensitivity to CsA.
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U2 - 10.1016/0192-0561(88)90081-1
DO - 10.1016/0192-0561(88)90081-1
M3 - Article
C2 - 2972633
AN - SCOPUS:0023762302
VL - 10
SP - 619
EP - 625
JO - International Journal of Immunopharmacology
JF - International Journal of Immunopharmacology
SN - 0192-0561
IS - 5
ER -