Effects of de-alcoholised wines with different polyphenol content on DNA oxidative damage, gene expression of peripheral lymphocytes, and haemorheology

An intervention study in post-menopausal women

Lisa Giovannelli, Vanessa Pitozzi, Cristina Luceri, Lucia Giannini, Simona Toti, Simonetta Salvini, Francesco Sera, Jean Marc Souquet, Veronique Cheynier, Francesco Sofi, Lucia Mannini, Anna Maria Gori, Rosanna Abbate, Domenico Palli, Piero Dolara

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose: Epidemiological studies suggest that a moderate consumption of wine is associated with a reduced risk of cardiovascular diseases and with a reduced mortality for all causes, possibly due to increased antioxidant defences. The present intervention study was undertaken to evaluate the in vivo effects of wine polyphenols on gene expression in humans, along with their supposed antioxidant activity. Methods: Blood haemorheology and platelet function were also evaluated. In order to avoid interferences from alcohol, we used de-alcoholised wine (DAW) with different polyphenol content. A randomised cross-over trial of high-proanthocyanidin (PA) red DAW (500 mL/die, PA dose = 7 mg/kg b.w.) vs. low-PA rosé DAW (500 mL/die, PA dose = 0.45 mg/kg) was conducted in 21 post-menopausal women in Florence, Italy. Oxidative DNA damage by the comet assay and gene expression by microarray was measured in peripheral blood lymphocytes, collected during the study period. Blood samples were also collected for the evaluation of haematological, haemostatic, haemorheological, and inflammatory parameters. Results: The results of the present study provide evidence that consumption of substantial amounts of de-alcoholised wine for 1 month does not exert a protective activity towards oxidative DNA damage, nor modifies significantly the gene expression profile of peripheral lymphocytes, whereas it shows blood-fluidifying actions, expressed as a significant decrease in blood viscosity. However, this effect does not correlate with the dosage of polyphenols of the de-alcoholised wine. Conclusions: More intervention studies are needed to provide further evidence of the health-protective effects of wine proanthocyanidins.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalEuropean Journal of Nutrition
Volume50
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Hemorheology
Polyphenols
Wine
DNA Damage
Lymphocytes
Gene Expression
Antioxidants
Proanthocyanidins
Blood Viscosity
Comet Assay
Hemostatics
Transcriptome
Cross-Over Studies
Italy
Epidemiologic Studies
Cardiovascular Diseases
Blood Platelets
Alcohols
Mortality
proanthocyanidin

Keywords

  • Comet assay
  • DNA damage
  • Microarray
  • Proanthocyanidins
  • Transcriptomics
  • Wine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Effects of de-alcoholised wines with different polyphenol content on DNA oxidative damage, gene expression of peripheral lymphocytes, and haemorheology : An intervention study in post-menopausal women. / Giovannelli, Lisa; Pitozzi, Vanessa; Luceri, Cristina; Giannini, Lucia; Toti, Simona; Salvini, Simonetta; Sera, Francesco; Souquet, Jean Marc; Cheynier, Veronique; Sofi, Francesco; Mannini, Lucia; Gori, Anna Maria; Abbate, Rosanna; Palli, Domenico; Dolara, Piero.

In: European Journal of Nutrition, Vol. 50, No. 1, 02.2011, p. 19-29.

Research output: Contribution to journalArticle

Giovannelli, L, Pitozzi, V, Luceri, C, Giannini, L, Toti, S, Salvini, S, Sera, F, Souquet, JM, Cheynier, V, Sofi, F, Mannini, L, Gori, AM, Abbate, R, Palli, D & Dolara, P 2011, 'Effects of de-alcoholised wines with different polyphenol content on DNA oxidative damage, gene expression of peripheral lymphocytes, and haemorheology: An intervention study in post-menopausal women', European Journal of Nutrition, vol. 50, no. 1, pp. 19-29. https://doi.org/10.1007/s00394-010-0111-1
Giovannelli, Lisa ; Pitozzi, Vanessa ; Luceri, Cristina ; Giannini, Lucia ; Toti, Simona ; Salvini, Simonetta ; Sera, Francesco ; Souquet, Jean Marc ; Cheynier, Veronique ; Sofi, Francesco ; Mannini, Lucia ; Gori, Anna Maria ; Abbate, Rosanna ; Palli, Domenico ; Dolara, Piero. / Effects of de-alcoholised wines with different polyphenol content on DNA oxidative damage, gene expression of peripheral lymphocytes, and haemorheology : An intervention study in post-menopausal women. In: European Journal of Nutrition. 2011 ; Vol. 50, No. 1. pp. 19-29.
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