Effects of dexamethasone on pro-inflammatory cytokine expression, cell growth and maturation during granulocytic differentiation of acute promyelocytic leukemia cells

M. Gianni, P. Norio, M. Terao, A. Falanga, M. Marchetti, A. Rambaldi, E. Garattini

Research output: Contribution to journalArticlepeer-review

Abstract

In basal conditions, NB4 and HL-60, two acute promyelocytic leukemia cell lines, express high levels of tumor necrosis factor (TNF-α) mRNA, whereas they do not synthesize appreciable amounts of the transcripts coding for interleukin-1β (IL-1β) or interleukin-8 (IL-8). Upon granulocytic differentiation with all-trans retinoic acid (ATRA) or the combination of ATRA and granulocyte-colony-stimulating factor (G-CSF), significant amounts of IL-1β and IL-8 mRNAs accumulated in both cell types. These changes in mRNA levels were parallelled by the increased secretion of the two cytokines. Dexamethasone (DEX) had no effect on the induction of IL-1β mRNA, while it enhanced the G-CSF-, ATRA- and (ATRA + G-CSF) dependent secretion of the cytokine. In combination with ATRA and G-CSF, the corticosteroid increased the expression of leukocyte alkaline phosphatase, a late marker of granulocytic differentiation.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalEuropean Cytokine Network
Volume6
Issue number3
Publication statusPublished - 1995

Keywords

  • Acute promyelocytic leukemia
  • Dexamethasone
  • IL-1
  • IL-8
  • TNF

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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