TY - JOUR
T1 - Effects of dexamethasone on pro-inflammatory cytokine expression, cell growth and maturation during granulocytic differentiation of acute promyelocytic leukemia cells
AU - Gianni, M.
AU - Norio, P.
AU - Terao, M.
AU - Falanga, A.
AU - Marchetti, M.
AU - Rambaldi, A.
AU - Garattini, E.
PY - 1995
Y1 - 1995
N2 - In basal conditions, NB4 and HL-60, two acute promyelocytic leukemia cell lines, express high levels of tumor necrosis factor (TNF-α) mRNA, whereas they do not synthesize appreciable amounts of the transcripts coding for interleukin-1β (IL-1β) or interleukin-8 (IL-8). Upon granulocytic differentiation with all-trans retinoic acid (ATRA) or the combination of ATRA and granulocyte-colony-stimulating factor (G-CSF), significant amounts of IL-1β and IL-8 mRNAs accumulated in both cell types. These changes in mRNA levels were parallelled by the increased secretion of the two cytokines. Dexamethasone (DEX) had no effect on the induction of IL-1β mRNA, while it enhanced the G-CSF-, ATRA- and (ATRA + G-CSF) dependent secretion of the cytokine. In combination with ATRA and G-CSF, the corticosteroid increased the expression of leukocyte alkaline phosphatase, a late marker of granulocytic differentiation.
AB - In basal conditions, NB4 and HL-60, two acute promyelocytic leukemia cell lines, express high levels of tumor necrosis factor (TNF-α) mRNA, whereas they do not synthesize appreciable amounts of the transcripts coding for interleukin-1β (IL-1β) or interleukin-8 (IL-8). Upon granulocytic differentiation with all-trans retinoic acid (ATRA) or the combination of ATRA and granulocyte-colony-stimulating factor (G-CSF), significant amounts of IL-1β and IL-8 mRNAs accumulated in both cell types. These changes in mRNA levels were parallelled by the increased secretion of the two cytokines. Dexamethasone (DEX) had no effect on the induction of IL-1β mRNA, while it enhanced the G-CSF-, ATRA- and (ATRA + G-CSF) dependent secretion of the cytokine. In combination with ATRA and G-CSF, the corticosteroid increased the expression of leukocyte alkaline phosphatase, a late marker of granulocytic differentiation.
KW - Acute promyelocytic leukemia
KW - Dexamethasone
KW - IL-1
KW - IL-8
KW - TNF
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M3 - Article
C2 - 8589272
AN - SCOPUS:0028807597
VL - 6
SP - 157
EP - 165
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 3
ER -