TY - JOUR
T1 - Effects of dopaminergic stimulation on peripheral markers of apoptosis
T2 - Relevance to Parkinson's disease
AU - Blandini, F.
AU - Mangiagalli, A.
AU - Martignoni, E.
AU - Samuele, A.
AU - Fancellu, R.
AU - Tassorelli, C.
AU - Cosentino, M.
AU - Marino, F.
AU - Rasini, E.
AU - Calandrella, D.
AU - Riboldazzi, G.
AU - Colombo, C.
AU - Frigo, G. M.
AU - Nappi, G.
PY - 2003/10
Y1 - 2003/10
N2 - We investigated the effects of dopaminergic stimulation on anti-apoptotic protein Bcl-2, pro-apoptotic enzyme caspase-3, and anti-oxidant/anti-apoptotic enzyme Cu/Zn super-oxide dismutase (SOD) in human lymphocytes exposed to dopamine (DA). The same determinations were also carried out in parkinsonian patients treated with L-dopa. Caspase-3 activity and Cu/Zn SOD levels tended to increase when lymphocytes were exposed to low or intermediate doses of DA, while a decrease was observed, particularly in caspase-3 activity, with the higher DA dose. Bcl-2 levels were unaffected. In patients, we observed a negative correlation between Cu/Zn SOD levels and daily intake of L-dopa, which also tended to be negatively correlated with caspase-3 activity, but not with Bcl-2. Our results show that dopaminergic stimulation is associated with complex changes in regulatory proteins of apoptosis.
AB - We investigated the effects of dopaminergic stimulation on anti-apoptotic protein Bcl-2, pro-apoptotic enzyme caspase-3, and anti-oxidant/anti-apoptotic enzyme Cu/Zn super-oxide dismutase (SOD) in human lymphocytes exposed to dopamine (DA). The same determinations were also carried out in parkinsonian patients treated with L-dopa. Caspase-3 activity and Cu/Zn SOD levels tended to increase when lymphocytes were exposed to low or intermediate doses of DA, while a decrease was observed, particularly in caspase-3 activity, with the higher DA dose. Bcl-2 levels were unaffected. In patients, we observed a negative correlation between Cu/Zn SOD levels and daily intake of L-dopa, which also tended to be negatively correlated with caspase-3 activity, but not with Bcl-2. Our results show that dopaminergic stimulation is associated with complex changes in regulatory proteins of apoptosis.
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U2 - 10.1007/s10072-003-0107-1
DO - 10.1007/s10072-003-0107-1
M3 - Article
C2 - 14598064
AN - SCOPUS:0142155265
VL - 24
SP - 157
EP - 158
JO - Neurological Sciences
JF - Neurological Sciences
SN - 1590-1874
IS - 3
ER -