TY - JOUR
T1 - Effects of electrical stimulation and tetrodotoxin paralysis on expression of muscle-specific isozymes of four enzymes in aneurally cultured embryonic rat muscle
AU - Rösler, Kai M.
AU - Askanas, Valerie
AU - Engel, W. King
AU - Martinuzzi, Andrea
PY - 1987
Y1 - 1987
N2 - We studied the effect of electrical stimulation and a sodium channel blocker (tetrodotoxin) on the expression of muscle-specific isozymes of creatine kinase, glycogen phosphorylase, phosphoglycerate mutase, and lactate dehydrogenase in aneurally cultured embryonic rat muscle. Muscle contractile activity slightly accelerated the accumulation of muscle-specific isozyme of creatine kinase in early cultures (4 days of experiment), but no increase in the expression of muscle-specific isozymes of any enzyme was present in older cultures (11 days of experiment). We conclude that muscle contractile activity is not a main regulator of isozyme maturation in this system.
AB - We studied the effect of electrical stimulation and a sodium channel blocker (tetrodotoxin) on the expression of muscle-specific isozymes of creatine kinase, glycogen phosphorylase, phosphoglycerate mutase, and lactate dehydrogenase in aneurally cultured embryonic rat muscle. Muscle contractile activity slightly accelerated the accumulation of muscle-specific isozyme of creatine kinase in early cultures (4 days of experiment), but no increase in the expression of muscle-specific isozymes of any enzyme was present in older cultures (11 days of experiment). We conclude that muscle contractile activity is not a main regulator of isozyme maturation in this system.
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U2 - 10.1016/0014-4886(87)90131-2
DO - 10.1016/0014-4886(87)90131-2
M3 - Article
C2 - 2957228
AN - SCOPUS:0023205863
VL - 97
SP - 739
EP - 745
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 3
ER -