Effects of electrical stimulation and tetrodotoxin paralysis on expression of muscle-specific isozymes of four enzymes in aneurally cultured embryonic rat muscle

Kai M. Rösler, Valerie Askanas, W. King Engel, Andrea Martinuzzi

Research output: Contribution to journalArticle

Abstract

We studied the effect of electrical stimulation and a sodium channel blocker (tetrodotoxin) on the expression of muscle-specific isozymes of creatine kinase, glycogen phosphorylase, phosphoglycerate mutase, and lactate dehydrogenase in aneurally cultured embryonic rat muscle. Muscle contractile activity slightly accelerated the accumulation of muscle-specific isozyme of creatine kinase in early cultures (4 days of experiment), but no increase in the expression of muscle-specific isozymes of any enzyme was present in older cultures (11 days of experiment). We conclude that muscle contractile activity is not a main regulator of isozyme maturation in this system.

Original languageEnglish
Pages (from-to)739-745
Number of pages7
JournalExperimental Neurology
Volume97
Issue number3
DOIs
Publication statusPublished - 1987

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

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