Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel.

E. Saino, L. Fassina, S. Van Vlierberghe, M. A. Avanzini, P. Dubruel, G. Magenes, L. Visai, F. Benazzo

Research output: Contribution to journalArticlepeer-review

Abstract

Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins. Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number1 Suppl 2
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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