TY - JOUR
T1 - Effects of encapsulated fruit and vegetable juice powder concentrates on oxidative status in heavy smokers
AU - Novembrino, Cristina
AU - Cighetti, Giuliana
AU - De Giuseppe, Rachele
AU - Vigna, Luisella
AU - de Liso, Federica
AU - Pellegatta, Marco
AU - Gregori, Dario
AU - Maiavacca, Rita
AU - Bamonti, Fabrizia
PY - 2011/2
Y1 - 2011/2
N2 - Objective: Long-term cigarette smoking has negative effects on oxidative status, promoting low-density lipoprotein (LDL) oxidation and formation of lipid peroxides. We evaluated the effects of 2 different encapsulated formulas, consisting primarily of mixed juice powder concentrate, on oxidative status compared with placebo. Methods: This randomized, double-blind, placebo-controlled study was performed on 101 apparently healthy heavy smokers (>20 cigarettes/d, duration >10 years; median age 47 years, range 41-57 years; 54 M) before and after 3 months' supplementation. Subjects were randomized into 3 groups, well matched for sex and age: (1) placebo; (2) fruit/vegetable (FV); and (3) fruit/vegetable/berry (FVB). Analysis of oxidative status was performed on 75 (46 M) compliant subjects (.95% of assigned capsules). Changes in lipid panel parameters, oxidative- INDEX (Oxy-I, calculated on the basis of serum hydroperoxides and total antioxidant capacity measured by spectrophotometric methods), oxidized-LDL (ox-LDL; enzyme-linked immunosorbent assay [ELISA] method), and malondialdehyde (MDA; gas chromatography-mass spectrometry method) in free (fMDA), bound (bMDA), and total (tMDA = fMDA {thorn} bMDA) forms are reported. Statistical analysis was performed with R statistical software. Results: After supplementation, compared with placebo, both FV and FVB groups showed a significant decrease in total cholesterol (p<0.05), ox-LDL (p = 0.03), and fMDA levels (p = 0.004) accompanied by a slight increase in bMDA concentrations, possibly as the result of fMDA conjugation. Moreover, a significant decrease in Oxy-I was found in both active groups compared with placebo (p, 0.001). Conclusion: Intervention with both nutraceutical formulations resulted in improvement in some oxidative alterations attributed to long-term cigarette smoking.
AB - Objective: Long-term cigarette smoking has negative effects on oxidative status, promoting low-density lipoprotein (LDL) oxidation and formation of lipid peroxides. We evaluated the effects of 2 different encapsulated formulas, consisting primarily of mixed juice powder concentrate, on oxidative status compared with placebo. Methods: This randomized, double-blind, placebo-controlled study was performed on 101 apparently healthy heavy smokers (>20 cigarettes/d, duration >10 years; median age 47 years, range 41-57 years; 54 M) before and after 3 months' supplementation. Subjects were randomized into 3 groups, well matched for sex and age: (1) placebo; (2) fruit/vegetable (FV); and (3) fruit/vegetable/berry (FVB). Analysis of oxidative status was performed on 75 (46 M) compliant subjects (.95% of assigned capsules). Changes in lipid panel parameters, oxidative- INDEX (Oxy-I, calculated on the basis of serum hydroperoxides and total antioxidant capacity measured by spectrophotometric methods), oxidized-LDL (ox-LDL; enzyme-linked immunosorbent assay [ELISA] method), and malondialdehyde (MDA; gas chromatography-mass spectrometry method) in free (fMDA), bound (bMDA), and total (tMDA = fMDA {thorn} bMDA) forms are reported. Statistical analysis was performed with R statistical software. Results: After supplementation, compared with placebo, both FV and FVB groups showed a significant decrease in total cholesterol (p<0.05), ox-LDL (p = 0.03), and fMDA levels (p = 0.004) accompanied by a slight increase in bMDA concentrations, possibly as the result of fMDA conjugation. Moreover, a significant decrease in Oxy-I was found in both active groups compared with placebo (p, 0.001). Conclusion: Intervention with both nutraceutical formulations resulted in improvement in some oxidative alterations attributed to long-term cigarette smoking.
KW - Cigarette smoking
KW - Lipid peroxidation
KW - Nutraceutical supplementation
KW - Oxidative status
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M3 - Article
C2 - 21697538
AN - SCOPUS:79960368577
VL - 30
SP - 49
EP - 56
JO - Journal of the American College of Nutrition
JF - Journal of the American College of Nutrition
SN - 0731-5724
IS - 1
ER -