Effects of environmental pollutants on signaling pathways in rat pituitary GH3 adenoma cells

Nicoletta Fortunati, Federica Guaraldi, Valentina Zunino, Federica Penner, Valentina D'Angelo, Francesco Zenga, Francesca Pecori Giraldi, Maria Graziella Catalano, Emanuela Arvat

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An increased rate of acromegaly was reported in industrialized areas, suggesting an involvement of environmental pollutants in the pathogenesis and behavior of GH-secreting pituitary adenomas. Based on these premises, the aim of the study was to evaluate the effects of some widely diffused pollutants (i.e. benzene, BZ; bis(2-ethylhexyl) phthalate, DEHP and polychlorinated biphenyls, PCB) on growth hormone secretion, the somatostatin and estrogenic pathways, viability and proliferation of rat GH-producing pituitary adenoma (GH3) cells. All the pollutants induced a statistically significant increase in GH secretion and interfered with cell signaling. They all modulated the expression of SSTR2 and ZAC1, involved in the somatostatin signaling, and the expression of the transcription factor FOXA1, involved in the estrogen receptor signaling. Moreover, all the pollutants increased the expression of the CYP1A1, suggesting AHR pathway activation. None of the pollutants impacted on cell proliferation or viability. Present data demonstrate that exposure to different pollutants, used at in vivo relevant concentrations, plays an important role in the behavior of GH3 pituitary adenoma cells, by increasing GH secretion and modulating several cellular signaling pathways. These observations support a possible influence of different pollutants in vivo on the GH-adenoma aggressiveness and biological behavior.

Original languageEnglish
Pages (from-to)660-668
Number of pages9
JournalEnvironmental Research
Volume158
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Diethylhexyl Phthalate
Cell signaling
Environmental Pollutants
Polychlorinated Biphenyls
Pituitary Neoplasms
Somatostatin
Rats
Cytochrome P-450 CYP1A1
pollutant
Cell proliferation
Growth Hormone-Secreting Pituitary Adenoma
Benzene
Estrogen Receptors
Growth Hormone
secretion
Acromegaly
Transcription Factors
Chemical activation
Adenoma
Cell Survival

Keywords

  • Benzene
  • DEHP
  • GH
  • PCB
  • Pituitary adenoma
  • Pollution
  • Somatostatin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

Cite this

Effects of environmental pollutants on signaling pathways in rat pituitary GH3 adenoma cells. / Fortunati, Nicoletta; Guaraldi, Federica; Zunino, Valentina; Penner, Federica; D'Angelo, Valentina; Zenga, Francesco; Pecori Giraldi, Francesca; Catalano, Maria Graziella; Arvat, Emanuela.

In: Environmental Research, Vol. 158, 01.10.2017, p. 660-668.

Research output: Contribution to journalArticle

Fortunati, Nicoletta ; Guaraldi, Federica ; Zunino, Valentina ; Penner, Federica ; D'Angelo, Valentina ; Zenga, Francesco ; Pecori Giraldi, Francesca ; Catalano, Maria Graziella ; Arvat, Emanuela. / Effects of environmental pollutants on signaling pathways in rat pituitary GH3 adenoma cells. In: Environmental Research. 2017 ; Vol. 158. pp. 660-668.
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