The effects of sterilization modalities on dialysis-induced cytokine release are still unknown. To investigate these effects, 8 patients on chronic hemodialysis were enrolled for evaluating at different intervals interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) production (pg/ml/106). They were using a 1.3 m2 ethylene oxide (E3) or steam (E3S) sterilized Cuprophan membrane. The patients underwent a basal test with E3 (A1) and 2 following tests after 1 (B1) and 2 (B2) months of E3S treatment, respectively. Finally, the last test was performed 1 month after the switch to E3 (A2). Il-1β predialysis release by mononuclear cells was 162 ± 114 pg/ml/106 in A1, 185 ± 129 pg/ml/106 in B1, and 226 ± 138 pg/ml/106 in B2, then decreased to 123 ± 134 in A2 (p <0.07). Il-1β postdialysis levels were 234 ± 238 pg/ml/106 in A1, 429 ± 285 pg/ml/106 (B1), and 438 ± 473 pg/ml/106 (B2) with the steam membrane, decreasing to 204 ± 134 pg/ml/106 in A2 (p <0.01). TNF-α predialysis basal release (A1) was 826 ± 817 pg/ml/106, 720 ± 496 in B1, and 1079 ± 515 pg/ml/106 in B2, and finally 680 ± 588 pg/ml/106in A2 (p <0.03). In postdialysis TNF-α levels were 963 ± 542 pg/ml/106 in A1, 1,226 ± 541 pg/ml/106, and 1,183 ± 776 in B1 and B2 respectively, and 388 ± 297 pg/ml/106 in A2 (p <0.003). Steam sterilization seems to induce a higher cytokine release by mononuclear cells when a Cuprophan membrane is used. This finding may be related to a less physiologic action of the steam in the case of Cuprophan membranes. Further studies are needed to clarify this hypothesis.
|Number of pages||3|
|Publication status||Published - 2002|
- Ethylene oxide
- Hemodialysis membrane
ASJC Scopus subject areas