Abstract
The side effects of ethilene oxide support the use of steam as the best sterilization procedure for medical devices. However, still now the effects of steam on mononuclear cytokine release, if any, are unknown. We enrolled 8 patients on chronic hemodialysis free from neoplastic, allergic or connective diseases, and compared the ETO and steam sterilization of cuprophan membrane (E3 and E3S, 1,3 m2 Fresenius AG). After a 3-month dialysis period with E3, a basal test was set up (A1); the same was performed after one-(B1) and two- (B2) month E3S treatment; finally, the last test was performed one month after the return to E3 (A2). In each test pre- and post dialysis samples were drawn for: LAL-test, to exclude dialysate contamination; IL-1β and TNF-α release after a 24-h incubation of mononuclear cells (MN) (ELISA pg/ml/106 MN); C3a and C5a levels at 0, 5', 15' and 60' to evaluate complement activation. LAL-test showed good quality of the dialysate through the study, and C3a and C5a levels were not different. Spontaneous release of IL-1β and TNF-α by MN was 162 and 826 pg/ml respectively in A1; it showed a clearly increase either in BI and B2 (185 and 226 for IL-1β and 720 and 1970 for TNF-α). The return to E3 showed a decrease in the cytokine release (123 and 689 pg/ml, respectively). Thus, steam sterilization seems to induce a higher cytokine release by MN when a cuprophan membrane is used. Dialysate contamination and a different complement activation being excluded, the only cause of the different cytokine production is the sterilization method. These results underlined the great complexity of the biological interactions of medical devices.
| Original language | Italian |
|---|---|
| Pages (from-to) | 249-254 |
| Number of pages | 6 |
| Journal | Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia |
| Volume | 15 |
| Issue number | 5 |
| Publication status | Published - 1998 |
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ASJC Scopus subject areas
- Nephrology
Cite this
Sterilizzazione del cuprophan con vapore ed ossido di etilene : Relazione con la produzione linfomonocitaria di citochine. / Aucella, F.; Vigilante, M.; Piemontese, M.; Grandone, E.; Colaizzo, D.; Margaglione, M.; Modoni, S. G.; Stallone, C.
In: Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia, Vol. 15, No. 5, 1998, p. 249-254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sterilizzazione del cuprophan con vapore ed ossido di etilene
T2 - Relazione con la produzione linfomonocitaria di citochine
AU - Aucella, F.
AU - Vigilante, M.
AU - Piemontese, M.
AU - Grandone, E.
AU - Colaizzo, D.
AU - Margaglione, M.
AU - Modoni, S. G.
AU - Stallone, C.
PY - 1998
Y1 - 1998
N2 - The side effects of ethilene oxide support the use of steam as the best sterilization procedure for medical devices. However, still now the effects of steam on mononuclear cytokine release, if any, are unknown. We enrolled 8 patients on chronic hemodialysis free from neoplastic, allergic or connective diseases, and compared the ETO and steam sterilization of cuprophan membrane (E3 and E3S, 1,3 m2 Fresenius AG). After a 3-month dialysis period with E3, a basal test was set up (A1); the same was performed after one-(B1) and two- (B2) month E3S treatment; finally, the last test was performed one month after the return to E3 (A2). In each test pre- and post dialysis samples were drawn for: LAL-test, to exclude dialysate contamination; IL-1β and TNF-α release after a 24-h incubation of mononuclear cells (MN) (ELISA pg/ml/106 MN); C3a and C5a levels at 0, 5', 15' and 60' to evaluate complement activation. LAL-test showed good quality of the dialysate through the study, and C3a and C5a levels were not different. Spontaneous release of IL-1β and TNF-α by MN was 162 and 826 pg/ml respectively in A1; it showed a clearly increase either in BI and B2 (185 and 226 for IL-1β and 720 and 1970 for TNF-α). The return to E3 showed a decrease in the cytokine release (123 and 689 pg/ml, respectively). Thus, steam sterilization seems to induce a higher cytokine release by MN when a cuprophan membrane is used. Dialysate contamination and a different complement activation being excluded, the only cause of the different cytokine production is the sterilization method. These results underlined the great complexity of the biological interactions of medical devices.
AB - The side effects of ethilene oxide support the use of steam as the best sterilization procedure for medical devices. However, still now the effects of steam on mononuclear cytokine release, if any, are unknown. We enrolled 8 patients on chronic hemodialysis free from neoplastic, allergic or connective diseases, and compared the ETO and steam sterilization of cuprophan membrane (E3 and E3S, 1,3 m2 Fresenius AG). After a 3-month dialysis period with E3, a basal test was set up (A1); the same was performed after one-(B1) and two- (B2) month E3S treatment; finally, the last test was performed one month after the return to E3 (A2). In each test pre- and post dialysis samples were drawn for: LAL-test, to exclude dialysate contamination; IL-1β and TNF-α release after a 24-h incubation of mononuclear cells (MN) (ELISA pg/ml/106 MN); C3a and C5a levels at 0, 5', 15' and 60' to evaluate complement activation. LAL-test showed good quality of the dialysate through the study, and C3a and C5a levels were not different. Spontaneous release of IL-1β and TNF-α by MN was 162 and 826 pg/ml respectively in A1; it showed a clearly increase either in BI and B2 (185 and 226 for IL-1β and 720 and 1970 for TNF-α). The return to E3 showed a decrease in the cytokine release (123 and 689 pg/ml, respectively). Thus, steam sterilization seems to induce a higher cytokine release by MN when a cuprophan membrane is used. Dialysate contamination and a different complement activation being excluded, the only cause of the different cytokine production is the sterilization method. These results underlined the great complexity of the biological interactions of medical devices.
KW - Cytokines
KW - Ethilene oxide
KW - Hemodialysis membrane
KW - Steam
KW - Sterilization
UR - http://www.scopus.com/inward/record.url?scp=0031737877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031737877&partnerID=8YFLogxK
M3 - Articolo
AN - SCOPUS:0031737877
VL - 15
SP - 249
EP - 254
JO - Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia
JF - Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia
SN - 0393-5590
IS - 5
ER -