Effects of exposure to gradient magnetic fields emitted by nuclear magnetic resonance devices on clonogenic potential and proliferation of human hematopoietic stem cells

Maria Grazia Iachininoto, Vincenzo Camisa, Lucia Leone, Rosanna Pinto, Vanni Lopresto, Caterina Merla, Ezio Giorda, Rita Carsetti, Salvatore Zaffina, Maria Vittoria Podda, Luciana Teofili, Claudio Grassi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study investigates effects of gradient magnetic fields (GMFs) emitted by magnetic resonance imaging (MRI) devices on hematopoietic stem cells. Field measurements were performed to assess exposure to GMFs of staff working at 1.5 T and 3 T MRI units. Then an exposure system reproducing measured signals was realized to expose in vitro CD34+ cells to GMFs (1.5 T-protocol and 3 T-protocol). CD34+ cells were obtained by Fluorescence Activated Cell Sorting from six blood donors and three MRI-exposed workers. Blood donor CD34+ cells were exposed in vitro for 72h to 1.5 T or 3 T-protocol and to sham procedure. Cells were then cultured and evaluated in colony forming unit (CFU)-assay up to 4 weeks after exposure. Results showed that in vitro GMF exposure did not affect cell proliferation but instead induced expansion of erythroid and monocytes progenitors soon after exposure and for the subsequent 3 weeks. No decrease of other clonogenic cell output (i.e., CFU-granulocyte/erythroid/macrophage/megakaryocyte and CFU-granulocyte/macrophage) was noticed, nor exposed CD34+ cells underwent the premature exhaustion of their clonogenic potential compared to sham-exposed controls. On the other hand, pilot experiments showed that CD34+ cells exposed in vivo to GMFs (i.e., samples from MRI workers) behaved in culture similarly to sham-exposed CD34+ cells, suggesting that other cells and/or microenvironment factors might prevent GMF effects on hematopoietic stem cells in vivo. Accordingly, GMFs did not affect the clonogenic potential of umbilical cord blood CD34+ cells exposed in vitro together with the whole mononuclear cell fraction.

Original languageEnglish
Pages (from-to)201-211
Number of pages11
JournalBioelectromagnetics
Volume37
Issue number4
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Magnetic Fields
Hematopoietic Stem Cells
Magnetic Resonance Spectroscopy
Equipment and Supplies
Magnetic Resonance Imaging
Blood Donors
Colony-Forming Units Assay
Myeloid Progenitor Cells
Cellular Microenvironment
Fetal Blood
Granulocytes
Monocytes
Blood Cells
Flow Cytometry
Stem Cells
Macrophages
Cell Proliferation
In Vitro Techniques

Keywords

  • CD34+ cells
  • Clonogenic potential
  • Complex magnetic field waveforms
  • Occupational exposure assessment

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Physiology

Cite this

Effects of exposure to gradient magnetic fields emitted by nuclear magnetic resonance devices on clonogenic potential and proliferation of human hematopoietic stem cells. / Iachininoto, Maria Grazia; Camisa, Vincenzo; Leone, Lucia; Pinto, Rosanna; Lopresto, Vanni; Merla, Caterina; Giorda, Ezio; Carsetti, Rita; Zaffina, Salvatore; Podda, Maria Vittoria; Teofili, Luciana; Grassi, Claudio.

In: Bioelectromagnetics, Vol. 37, No. 4, 01.05.2016, p. 201-211.

Research output: Contribution to journalArticle

Iachininoto, Maria Grazia ; Camisa, Vincenzo ; Leone, Lucia ; Pinto, Rosanna ; Lopresto, Vanni ; Merla, Caterina ; Giorda, Ezio ; Carsetti, Rita ; Zaffina, Salvatore ; Podda, Maria Vittoria ; Teofili, Luciana ; Grassi, Claudio. / Effects of exposure to gradient magnetic fields emitted by nuclear magnetic resonance devices on clonogenic potential and proliferation of human hematopoietic stem cells. In: Bioelectromagnetics. 2016 ; Vol. 37, No. 4. pp. 201-211.
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AU - Lopresto, Vanni

AU - Merla, Caterina

AU - Giorda, Ezio

AU - Carsetti, Rita

AU - Zaffina, Salvatore

AU - Podda, Maria Vittoria

AU - Teofili, Luciana

AU - Grassi, Claudio

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