Effects of folic acid before and after vitamin B12 on plasma homocysteine concentrations in hemodialysis patients with known MTHFR genotypes

Anna Pastore, Sandra De Angelis, Stefania Casciani, Rosalba Ruggia, Gianna Di Giovamberardino, Annalisa Noce, Giorgio Splendiani, Claudio Cortese, Giorgio Federici, Mariarita Dessì

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Treatment with folic acid and vitamin B12 appears to be effective in lowering total plasma homocysteine (tHcy) concentrations, but whether vitamin B12 alone lowers tHcy in patients with normal vitamin B12 status is unknown. The aims of the present study were to explore the effect of individual supplementation with folic acid or vitamin B 12 on tHcy concentrations in hemodialysis (HD) patients and to compare changes in tHcy concentrations with MTHFR genotype. Methods: We recruited 200 HD patients (119 men) from the "Umberto I" Hospital (Frosinone, Italy) and the Dialysis Unit of University Hospital "Tor Vergata". These patients were randomized blindly into 2 groups of 100 each. Unfortunately, during the study, 36 patients in the first group and 16 in the second group died. The first group was treated initially with vitamin B 12 for 2 months and with folic acid for a following 2 months. The second group was treated initially with folic acid and then with vitamin B 12. Samples were drawn before administration of either, after the first and second periods, and again 2 months after treatment. Results: The concentrations of tHcy decreased in both groups after the consecutive vitamin therapies, and the decrease was genotype-dependent. The decrease was greater for the T/T genotype (P 12 in lowering tHcy in HD patients.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalClinical Chemistry
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2006

ASJC Scopus subject areas

  • Clinical Biochemistry

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