Effects of genistein on the polyamine metabolism and cell growth in DLD-1 human colon cancer cells

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Abstract

Polyamines and their rate-limiting enzyme, ornithine decarboxylase (ODC), are actively involved in cell growth and differentiation. The phytoestrogen genistein has been demonstrated to possess antitumor properties by influencing proliferation, differentiation, and apoptosis. The aim of this study was to investigate the effects of genistein at concentrations ranging from 0.01 to 100 μM on the polyamine biosynthesis, cell proliferation, and apoptosis in the estrogen receptor-positive DLD-1 human colon cancer cell line. Polyamine levels and ODC activity were evaluated by high performance liquid chromatography and radiometric technique, respectively. The proliferative response was estimated by [3H]-thymidine incorporation and the colorimetric 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Apoptosis was investigated by DNA fragmentation. Box and Bcl-2 gene expressions were evaluated by multiplex-polymerase chain reaction. At concentration ≥1 μM, genistein decreased significantly the ODC activity and the polyamine levels. At the same concentration, genistein also increased significantly Box mRNA expression, but not Bcl-2 mRNA expression. Higher concentrations (≥10 μM) were needed to obtain a significant inhibition of cell proliferation and DNA fragmentation. The results of this study suggest that genistein can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. However, further studies are required to assess the true ability of a soy rich diet in modifying colon cancer risk.

Original languageEnglish
Pages (from-to)84-93
Number of pages10
JournalNutrition and Cancer
Volume52
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Genistein
genistein
Polyamines
polyamines
colorectal neoplasms
Colonic Neoplasms
cell growth
ornithine decarboxylase
Ornithine Decarboxylase
apoptosis
metabolism
Apoptosis
Growth
DNA fragmentation
DNA Fragmentation
cell proliferation
Cell Proliferation
biosynthesis
bcl-2 Genes
Phytoestrogens

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Oncology

Cite this

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title = "Effects of genistein on the polyamine metabolism and cell growth in DLD-1 human colon cancer cells",
abstract = "Polyamines and their rate-limiting enzyme, ornithine decarboxylase (ODC), are actively involved in cell growth and differentiation. The phytoestrogen genistein has been demonstrated to possess antitumor properties by influencing proliferation, differentiation, and apoptosis. The aim of this study was to investigate the effects of genistein at concentrations ranging from 0.01 to 100 μM on the polyamine biosynthesis, cell proliferation, and apoptosis in the estrogen receptor-positive DLD-1 human colon cancer cell line. Polyamine levels and ODC activity were evaluated by high performance liquid chromatography and radiometric technique, respectively. The proliferative response was estimated by [3H]-thymidine incorporation and the colorimetric 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Apoptosis was investigated by DNA fragmentation. Box and Bcl-2 gene expressions were evaluated by multiplex-polymerase chain reaction. At concentration ≥1 μM, genistein decreased significantly the ODC activity and the polyamine levels. At the same concentration, genistein also increased significantly Box mRNA expression, but not Bcl-2 mRNA expression. Higher concentrations (≥10 μM) were needed to obtain a significant inhibition of cell proliferation and DNA fragmentation. The results of this study suggest that genistein can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. However, further studies are required to assess the true ability of a soy rich diet in modifying colon cancer risk.",
author = "Michele Linsalata and Francesco Russo and Maria Notarnicola and Vito Guerra and Aldo Cavallini and Caterina Clemente and Caterina Messa",
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T1 - Effects of genistein on the polyamine metabolism and cell growth in DLD-1 human colon cancer cells

AU - Linsalata, Michele

AU - Russo, Francesco

AU - Notarnicola, Maria

AU - Guerra, Vito

AU - Cavallini, Aldo

AU - Clemente, Caterina

AU - Messa, Caterina

PY - 2005

Y1 - 2005

N2 - Polyamines and their rate-limiting enzyme, ornithine decarboxylase (ODC), are actively involved in cell growth and differentiation. The phytoestrogen genistein has been demonstrated to possess antitumor properties by influencing proliferation, differentiation, and apoptosis. The aim of this study was to investigate the effects of genistein at concentrations ranging from 0.01 to 100 μM on the polyamine biosynthesis, cell proliferation, and apoptosis in the estrogen receptor-positive DLD-1 human colon cancer cell line. Polyamine levels and ODC activity were evaluated by high performance liquid chromatography and radiometric technique, respectively. The proliferative response was estimated by [3H]-thymidine incorporation and the colorimetric 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Apoptosis was investigated by DNA fragmentation. Box and Bcl-2 gene expressions were evaluated by multiplex-polymerase chain reaction. At concentration ≥1 μM, genistein decreased significantly the ODC activity and the polyamine levels. At the same concentration, genistein also increased significantly Box mRNA expression, but not Bcl-2 mRNA expression. Higher concentrations (≥10 μM) were needed to obtain a significant inhibition of cell proliferation and DNA fragmentation. The results of this study suggest that genistein can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. However, further studies are required to assess the true ability of a soy rich diet in modifying colon cancer risk.

AB - Polyamines and their rate-limiting enzyme, ornithine decarboxylase (ODC), are actively involved in cell growth and differentiation. The phytoestrogen genistein has been demonstrated to possess antitumor properties by influencing proliferation, differentiation, and apoptosis. The aim of this study was to investigate the effects of genistein at concentrations ranging from 0.01 to 100 μM on the polyamine biosynthesis, cell proliferation, and apoptosis in the estrogen receptor-positive DLD-1 human colon cancer cell line. Polyamine levels and ODC activity were evaluated by high performance liquid chromatography and radiometric technique, respectively. The proliferative response was estimated by [3H]-thymidine incorporation and the colorimetric 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Apoptosis was investigated by DNA fragmentation. Box and Bcl-2 gene expressions were evaluated by multiplex-polymerase chain reaction. At concentration ≥1 μM, genistein decreased significantly the ODC activity and the polyamine levels. At the same concentration, genistein also increased significantly Box mRNA expression, but not Bcl-2 mRNA expression. Higher concentrations (≥10 μM) were needed to obtain a significant inhibition of cell proliferation and DNA fragmentation. The results of this study suggest that genistein can affect growth of DLD-1 cells by both decreasing polyamine biosynthesis and inducing apoptosis. However, further studies are required to assess the true ability of a soy rich diet in modifying colon cancer risk.

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