Abstract
Two recombinant human granulocyte colony-stimulating factors (rHu G-CSF) are clinically available, a glycosylated (lenograstim) and a nonglycosylated form (filgrastim). Since there is accumulating evidence that glycosylation plays a role in the vitro activity of the G-CSF molecule, we compared the biological potency of lenograstim and filgrastim on human hematopietic progenitor cells by colony assay in semisolid medium and ex vivo expansion experiments. Leukapheretic products without further processing and CD34-positive purified cells were used as source of human progenitors. Lenograstim demonstrated greater capacity, to stimulate the colony growth of both, purified and CD34+ peripheral blood cells. This effects, which is evident especially at low doses of growth factor, seems not to be mediated by accessory cells. Whether these observations may have clinical relevance is still to be clearly assessed and further investigations are needed.
| Original language | English |
|---|---|
| Pages (from-to) | 1781-1785 |
| Number of pages | 5 |
| Journal | Anticancer Research |
| Volume | 16 |
| Issue number | 4 A |
| Publication status | Published - Jul 1996 |
Fingerprint
Keywords
- Hematopoietic progenitor cells
- Recombinant human G-CSF
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Effects of glycosylated and non-glycosylated G-CSFs, alone and in combination with other cytokines, on the growth of human progenitor cells. / Pedrazzoli, Paolo; Gibelli, Nadia; Pavesi, Lorenzo; Preti, Pietro; Piolini, Marco; Bertolini, Francesco; Robustelli Della Cuna, Gioacchino.
In: Anticancer Research, Vol. 16, No. 4 A, 07.1996, p. 1781-1785.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of glycosylated and non-glycosylated G-CSFs, alone and in combination with other cytokines, on the growth of human progenitor cells
AU - Pedrazzoli, Paolo
AU - Gibelli, Nadia
AU - Pavesi, Lorenzo
AU - Preti, Pietro
AU - Piolini, Marco
AU - Bertolini, Francesco
AU - Robustelli Della Cuna, Gioacchino
PY - 1996/7
Y1 - 1996/7
N2 - Two recombinant human granulocyte colony-stimulating factors (rHu G-CSF) are clinically available, a glycosylated (lenograstim) and a nonglycosylated form (filgrastim). Since there is accumulating evidence that glycosylation plays a role in the vitro activity of the G-CSF molecule, we compared the biological potency of lenograstim and filgrastim on human hematopietic progenitor cells by colony assay in semisolid medium and ex vivo expansion experiments. Leukapheretic products without further processing and CD34-positive purified cells were used as source of human progenitors. Lenograstim demonstrated greater capacity, to stimulate the colony growth of both, purified and CD34+ peripheral blood cells. This effects, which is evident especially at low doses of growth factor, seems not to be mediated by accessory cells. Whether these observations may have clinical relevance is still to be clearly assessed and further investigations are needed.
AB - Two recombinant human granulocyte colony-stimulating factors (rHu G-CSF) are clinically available, a glycosylated (lenograstim) and a nonglycosylated form (filgrastim). Since there is accumulating evidence that glycosylation plays a role in the vitro activity of the G-CSF molecule, we compared the biological potency of lenograstim and filgrastim on human hematopietic progenitor cells by colony assay in semisolid medium and ex vivo expansion experiments. Leukapheretic products without further processing and CD34-positive purified cells were used as source of human progenitors. Lenograstim demonstrated greater capacity, to stimulate the colony growth of both, purified and CD34+ peripheral blood cells. This effects, which is evident especially at low doses of growth factor, seems not to be mediated by accessory cells. Whether these observations may have clinical relevance is still to be clearly assessed and further investigations are needed.
KW - Hematopoietic progenitor cells
KW - Recombinant human G-CSF
UR - http://www.scopus.com/inward/record.url?scp=0029759836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029759836&partnerID=8YFLogxK
M3 - Article
C2 - 8712701
AN - SCOPUS:0029759836
VL - 16
SP - 1781
EP - 1785
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 4 A
ER -