Effects of glycosylated and non-glycosylated G-CSFs, alone and in combination with other cytokines, on the growth of human progenitor cells

Paolo Pedrazzoli, Nadia Gibelli, Lorenzo Pavesi, Pietro Preti, Marco Piolini, Francesco Bertolini, Gioacchino Robustelli Della Cuna

Research output: Contribution to journalArticle

Abstract

Two recombinant human granulocyte colony-stimulating factors (rHu G-CSF) are clinically available, a glycosylated (lenograstim) and a nonglycosylated form (filgrastim). Since there is accumulating evidence that glycosylation plays a role in the vitro activity of the G-CSF molecule, we compared the biological potency of lenograstim and filgrastim on human hematopietic progenitor cells by colony assay in semisolid medium and ex vivo expansion experiments. Leukapheretic products without further processing and CD34-positive purified cells were used as source of human progenitors. Lenograstim demonstrated greater capacity, to stimulate the colony growth of both, purified and CD34+ peripheral blood cells. This effects, which is evident especially at low doses of growth factor, seems not to be mediated by accessory cells. Whether these observations may have clinical relevance is still to be clearly assessed and further investigations are needed.

Original languageEnglish
Pages (from-to)1781-1785
Number of pages5
JournalAnticancer Research
Volume16
Issue number4 A
Publication statusPublished - Jul 1996

Keywords

  • Hematopoietic progenitor cells
  • Recombinant human G-CSF

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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