Abstract
Nonhematopoietic malignant cells may express receptors for hematopoietic growth factors and respond to these peptides. The aim of the present study was to investigate whether small cell lung cancer (SCLC) cells may be stimulated to proliferate by hematopoietic growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), which are currently used in clinical trials in combination with cytotoxic chemotherapy. We studied two SCLC cell lines, H69 and N417. The effects of GM-CSF and IL-3 were evaluated by studying clonal growth, 3H-thymidine incorporation, BUDR/DNA bivariate flow cytometry, c-myc and N-myc oncogene expression, and myeloid surface markers. Our experiments show that both GM-CSF and IL-3 can increase 3H-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Further in vitro and in vivo studies are needed to determine whether clinical trials applying these factors for bone marrow recovery after chemotherapy of solid tumors may be hazardous by potentially stimulating growth of remaining tumor tissue.
| Original language | English |
|---|---|
| Pages (from-to) | 283-288 |
| Number of pages | 6 |
| Journal | Cancer Investigation |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Effects of granulocyte-macrophage colony-stimulating factor and interleukin-3 on small cell lung cancer cells. / Pedrazzoli, Paolo; Bacciocchi, Giovanna; Bergamaschi, Gaetano; Cazzola, Mario; Danova, Marco; Gibelli, Nadia; Giordano, Monica; Lazzaro, Antonio; Locatelli, Franco; Pavesi, Lorenzo; Volpato, Gino; Zibera, Carlo; Cuna, Gioacchino Robustelli Della.
In: Cancer Investigation, Vol. 12, No. 3, 1994, p. 283-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of granulocyte-macrophage colony-stimulating factor and interleukin-3 on small cell lung cancer cells
AU - Pedrazzoli, Paolo
AU - Bacciocchi, Giovanna
AU - Bergamaschi, Gaetano
AU - Cazzola, Mario
AU - Danova, Marco
AU - Gibelli, Nadia
AU - Giordano, Monica
AU - Lazzaro, Antonio
AU - Locatelli, Franco
AU - Pavesi, Lorenzo
AU - Volpato, Gino
AU - Zibera, Carlo
AU - Cuna, Gioacchino Robustelli Della
PY - 1994
Y1 - 1994
N2 - Nonhematopoietic malignant cells may express receptors for hematopoietic growth factors and respond to these peptides. The aim of the present study was to investigate whether small cell lung cancer (SCLC) cells may be stimulated to proliferate by hematopoietic growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), which are currently used in clinical trials in combination with cytotoxic chemotherapy. We studied two SCLC cell lines, H69 and N417. The effects of GM-CSF and IL-3 were evaluated by studying clonal growth, 3H-thymidine incorporation, BUDR/DNA bivariate flow cytometry, c-myc and N-myc oncogene expression, and myeloid surface markers. Our experiments show that both GM-CSF and IL-3 can increase 3H-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Further in vitro and in vivo studies are needed to determine whether clinical trials applying these factors for bone marrow recovery after chemotherapy of solid tumors may be hazardous by potentially stimulating growth of remaining tumor tissue.
AB - Nonhematopoietic malignant cells may express receptors for hematopoietic growth factors and respond to these peptides. The aim of the present study was to investigate whether small cell lung cancer (SCLC) cells may be stimulated to proliferate by hematopoietic growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), which are currently used in clinical trials in combination with cytotoxic chemotherapy. We studied two SCLC cell lines, H69 and N417. The effects of GM-CSF and IL-3 were evaluated by studying clonal growth, 3H-thymidine incorporation, BUDR/DNA bivariate flow cytometry, c-myc and N-myc oncogene expression, and myeloid surface markers. Our experiments show that both GM-CSF and IL-3 can increase 3H-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Further in vitro and in vivo studies are needed to determine whether clinical trials applying these factors for bone marrow recovery after chemotherapy of solid tumors may be hazardous by potentially stimulating growth of remaining tumor tissue.
UR - http://www.scopus.com/inward/record.url?scp=0028340802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028340802&partnerID=8YFLogxK
U2 - 10.3109/07357909409023026
DO - 10.3109/07357909409023026
M3 - Article
C2 - 8187006
AN - SCOPUS:0028340802
VL - 12
SP - 283
EP - 288
JO - Cancer Investigation
JF - Cancer Investigation
SN - 0735-7907
IS - 3
ER -