Effects of high and low pH on Ca(i)2+ and on cell injury evoked by anoxia in rat hepatocytes

A. Gasbarrini, A. B. Borle, P. Caraceni, A. Gasbarrini, G. Sica, A. Facchini, M. Bernardi, D. H. Van Thiel

Research output: Contribution to journalArticle

Abstract

The effect of high and low extracellular pH was studied in freshly isolated rat hepatocytes exposed to anoxia. Reducing the extracellular pH to 6.8 during the anoxia period decreased the intracellular pH to between 6.2 and 6.5; it did not reduce the rise in Ca(i)2+, but it abolished the LDH release. pH(o) increasing to 8.0 during anoxia increased pH(i) above 8.0 and doubled the LDH release without significantly altering the rise in Ca(i)2+. When fructose was present with a pH(o) of 8.0, pH(i) was still 8.0, but there was practically no rise in Ca(i)2+, and the LDH release was again completely abolished. These experiments suggest that a low pH prevents the activation of hydrolytic enzymes even in the presence of a high Ca(i)2+. Conversely, a high pH(i) can activate hydrolytic enzymes and cause cell injury even in the absence of an elevated Ca(i)2+. The mechanism by which fructose protects hepatocytes against anoxic cell injury at pH(i) 8.0 is unclear.

Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalTransplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft
Volume6
Issue number2
Publication statusPublished - 1994

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Hepatocytes
Wounds and Injuries
Fructose
Hypoxia
Enzyme Activation
Enzymes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation

Cite this

Effects of high and low pH on Ca(i)2+ and on cell injury evoked by anoxia in rat hepatocytes. / Gasbarrini, A.; Borle, A. B.; Caraceni, P.; Gasbarrini, A.; Sica, G.; Facchini, A.; Bernardi, M.; Van Thiel, D. H.

In: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, Vol. 6, No. 2, 1994, p. 166-170.

Research output: Contribution to journalArticle

Gasbarrini, A, Borle, AB, Caraceni, P, Gasbarrini, A, Sica, G, Facchini, A, Bernardi, M & Van Thiel, DH 1994, 'Effects of high and low pH on Ca(i)2+ and on cell injury evoked by anoxia in rat hepatocytes', Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, vol. 6, no. 2, pp. 166-170.
Gasbarrini, A. ; Borle, A. B. ; Caraceni, P. ; Gasbarrini, A. ; Sica, G. ; Facchini, A. ; Bernardi, M. ; Van Thiel, D. H. / Effects of high and low pH on Ca(i)2+ and on cell injury evoked by anoxia in rat hepatocytes. In: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft. 1994 ; Vol. 6, No. 2. pp. 166-170.
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AU - Gasbarrini, A.

AU - Sica, G.

AU - Facchini, A.

AU - Bernardi, M.

AU - Van Thiel, D. H.

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