Effects of hispolon on glioblastoma cell growth

A. Arcella, M.A. Oliva, M. Sanchez, S. Staffieri, V. Esposito, F. Giangaspero, G. Cantore

Research output: Contribution to journalArticlepeer-review

Abstract

Hispolon is a polyphenolic compound isolated from Phellinus linteus which exhibits antitumor activity. Here, we explored the effects of hispolon on human glioblastoma cells U87MG. Cell viability was examined by MTT assay. Growth was investigated by incubating cells with various concentrations of hispolon (25 and 50 µM) for 24, 48 or 72 h and daily cell count. Cell cycle and apoptosis assay were assessed by flow cytometry. Hispolon decreased cell viability in a dose- and time-dependent manner. The cell cycle distribution showed that hispolon enhanced the accumulation of the cells in G2/M phase. Hispolon decreased the expression of G1–S transition-related protein cyclin D4 but increased the expression of CDK inhibitor p21. Additionally, hispolon enhanced the expression of p53. Moreover, hispolon treatment was effective on U87MG cells in inhibiting cell viability and inducing cell apoptosis. Our results indicate that hispolon inhibits the cell viability, induces G2/M cell cycle arrest and apoptosis in glioblastoma U87MG cells, and p53 should play a role in hispolon-mediated antitumor activity. © 2017 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)2113-2123
Number of pages11
JournalEnvironmental Toxicology
Volume32
Issue number9
DOIs
Publication statusPublished - 2017

Keywords

  • adjuvant chemotherapy
  • apoptosis
  • basidiomycete
  • brain cancer
  • cell cycle
  • glioblastoma
  • hispolon
  • natural drug
  • Phellinus linteus
  • temozolomide
  • Cell death
  • Chemotherapy
  • Cytology
  • Polyphenolic compounds
  • Adjuvant chemotherapy
  • Basidiomycete
  • Brain cancer
  • Cell cycle
  • Glioblastomas
  • Hispolon
  • Natural drug
  • Temozolomide
  • Cells
  • antineoplastic agent
  • caspase 3
  • cyclin dependent kinase 4
  • polyphenol derivative
  • protein p21
  • protein p53
  • unclassified drug
  • catechol derivative
  • cyclin dependent kinase inhibitor 1A
  • TP53 protein, human
  • bioassay
  • brain
  • cancer
  • cells and cell components
  • chemotrophy
  • concentration (composition)
  • drug
  • flow cytometry
  • fungus
  • gene expression
  • growth
  • phenolic compound
  • protein
  • antineoplastic activity
  • Article
  • cancer growth
  • cancer inhibition
  • cell invasion
  • cell migration
  • cell proliferation
  • cell viability
  • concentration response
  • controlled study
  • drug potentiation
  • enzyme activation
  • G2 phase cell cycle checkpoint
  • glioblastoma cell line
  • human
  • human cell
  • in vitro study
  • priority journal
  • protein expression
  • protein phosphorylation
  • brain tumor
  • cell survival
  • drug effects
  • metabolism
  • tumor cell line
  • Inonotus linteus
  • Antineoplastic Agents
  • Apoptosis
  • Brain Neoplasms
  • Catechols
  • Cell Cycle
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival
  • Cyclin-Dependent Kinase Inhibitor p21
  • Glioblastoma
  • Humans
  • Tumor Suppressor Protein p53

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