The effects of the in vivo administration of a synthetic analogue of prostaglandin E2 (PGE2), 16,16-dimethyl-PGE2 (di-M-PGE2), on interleukin 2 (IL 2) production by splenocytes collected from normal and B 16 melanoma tumor-bearing mice have been analyzed. Splenocytes from normal and tumor-bearing mice were stimulated in vitro by concanavalin A and phorbol myristic acetate for 48 hours. The amount of IL 2 released in supernatants was measured by their ability to support the growth of an IL-2 dependent murine T cells line (CTLL). Results show that in vivo di-M-PGE2 administration is able to inhibit in a dose-dependent fashion the in vitro IL 2 production by spleen cells collected from normal mice, whereas it increases the IL 2 production from splenocytes collected from tumor-bearing mice. In order to clarify how PGE2 could affect IL 2 production, quantitative modifications of lymphocyte subpopulations have been studied. Flow cytometry analysis showed a slight reduction of total Thy 1.2+ splenocytes in normal mice after di-M-PGE2 administration. Moreover, the same percentage was markedly reduced during tumor growth and partially restored after di-M-PGE2 administration in tumor-bearing mice. Slight quantitative modifications regarding the L3T4+ and Lyt 2+ populations were observed. These data suggest that qualitative rather than quantitative changes in IL 2-producing cells are involved in the observed phenomenon.
|Number of pages||10|
|Journal||International Journal of Immunopathology and Pharmacology|
|Publication status||Published - 1989|
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