Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo

M. Marabese, S. Marchini, M. A. Sabatino, F. Polato, F. Vikhanskaya, E. Marrazzo, E. Riccardi, E. Scanziani, M. Broggini

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The p73 gene has a complex regulation, which leads to the expression of different isoforms, often with opposite biological effects. We have generated in the human colocarcinoma cell line HCT116, expressing a wild-type p53, an inducible DNp73α expressing system. Two clones (HCT116/DN3 and HCT116/DN14), upon doxycycline addition, show a strong expression of DNp73α. In vitro the two DNp73α overexpressing clones grow at similar rate of the control transfected clone (HCT116/8a) and similarly respond to DNA damage. When injected in mice, HCT116/DN3, HCT116/DN14, and HCT116/8a cells grew similarly in the absence or presence of tetracycline. In HCT116/DN3 and HCT116/DN14 tumors, tetracycline induced a strong expression of DNp73α both as mRNA and protein. These results indicate that in this system the overexpression of the DNp73α does not induce a more aggressive phenotype and does not seem to be associated with a reduced response of the cells to treatment with anticancer agents.

Original languageEnglish
Pages (from-to)805-814
Number of pages10
JournalCell Death and Differentiation
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Clone Cells
Tetracycline
Growth
HCT116 Cells
Neoplasms
Doxycycline
Antineoplastic Agents
DNA Damage
Protein Isoforms
Phenotype
Cell Line
Messenger RNA
Genes
In Vitro Techniques
Proteins

Keywords

  • Anticancer agents
  • In vivo
  • p53
  • p73

ASJC Scopus subject areas

  • Cell Biology

Cite this

Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo. / Marabese, M.; Marchini, S.; Sabatino, M. A.; Polato, F.; Vikhanskaya, F.; Marrazzo, E.; Riccardi, E.; Scanziani, E.; Broggini, M.

In: Cell Death and Differentiation, Vol. 12, No. 7, 07.2005, p. 805-814.

Research output: Contribution to journalArticle

Marabese, M. ; Marchini, S. ; Sabatino, M. A. ; Polato, F. ; Vikhanskaya, F. ; Marrazzo, E. ; Riccardi, E. ; Scanziani, E. ; Broggini, M. / Effects of inducible overexpression of DNp73α on cancer cell growth and response to treatment in vitro and in vivo. In: Cell Death and Differentiation. 2005 ; Vol. 12, No. 7. pp. 805-814.
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