Effects of interleukin-2 therapy on the proliferation and differentiation of CD4/CD25 positive and CD4/CD25 negative cells in HIV+ patients

L. Caggiari, S. Zanussi, M. D'Andrea, M. T. Bortolin, C. Crepaldi, C. Caffau, P. De Paoli

Research output: Contribution to journalArticle

Abstract

Interleukin-2 has been widely used in HIV-1+ subjects as an immunoactivating agent. In this study, we investigated cytokine production, Ki67 antigen expression and the modulation of the surface phenotype of the CD4/CD25+ subset as compared to the reciprocal CD4/CD25- subset in IL-2-treated HIV+ patients. Our findings suggest that CD4 T cells are heterogeneous in responding to IL-2, because CD4/CD25+ cells sharply increased their "memory" phenotype, their Ki67 antigen expression and were the main in vivo targets for IL-2-dependent proliferation during therapy, while the percentages of IFN-γ+ (terminally differentiated) cells remained unchanged at the end of therapy. Conversely, the CD4+/CD25- subpopulation showed an expansion of differentiated cells and a slight increase in the proliferation rate. The use of anti-retroviral therapy alone (HAART) reduced the proliferation and increased the differentiation of both CD4 subsets. Our data suggest that IL-2 has a moderate capacity to activate resting T cells in vivo and is probably unable to boost HIV-1 from latency to the replicative state.

Original languageEnglish
Pages (from-to)430-436
Number of pages7
JournalEuropean Cytokine Network
Volume12
Issue number3
Publication statusPublished - 2001

Keywords

  • Cytokines
  • Intracellular IFN-γ
  • Ki67

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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    Caggiari, L., Zanussi, S., D'Andrea, M., Bortolin, M. T., Crepaldi, C., Caffau, C., & De Paoli, P. (2001). Effects of interleukin-2 therapy on the proliferation and differentiation of CD4/CD25 positive and CD4/CD25 negative cells in HIV+ patients. European Cytokine Network, 12(3), 430-436.