Effects of L-DOPA Pretreatment on the Kinetics, Migration and Carbon Ion Radiation Response of T98G Cells

Angelica Facoetti, Miriam Cavagnini, Mario Ciocca, Rosanna Nano, Francesca Pasi, Carlo Aprile, Lorenzo Lodola, Marco Giovanni Persico, Manuela Marenco, Francesca Valvo, Roberto Orecchia

Research output: Contribution to journalArticle

Abstract

BACKGROUND/AIM: Glioblastoma is the most malignant and widespread brain tumor in adults, with a rapid clinical course. Recently, it has been hypothesized that L-DOPA plays a role in the diagnosis and treatment of glioblastoma. The aim of this study was to assess the effects of pretreatment with L-DOPA on the biological behavior of human T98G cells in vitro. MATERIALS AND METHODS: T98G cells were treated with 50 μg/ml or 100 μg/ml of L-DOPA for 4 h and their morphology, growth rate, clonogenic survival and migratory capacity in basal conditions and after carbon ion irradiation were evaluated using standard methods. RESULTS: Treated cells showed a lower growth rate and an increased migratory capacity that correlated with the dose of tested L-DOPA. Treatment with L-DOPA increased the growth rate of carbon ion irradiated T98G cells compared to control non-treated cells exposed to the same radiation dose. CONCLUSION: Our results open further questions about the overall advantage of L-DOPA treatment of glioblastoma.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalAnticancer Research
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • 18F-DOPA
  • carbon ion radiation
  • Glioblastoma
  • L-DOPA
  • migration
  • proliferation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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