Effects of L-DOPA pretreatment on the kinetics, migration and carbon ion radiation response of T98G cells: Anticancer Research

A. Facoetti, M. Cavagnini, M. Ciocca, R. Nano, F. Pasi, C. Aprile, L. Lodola, M.G. Persico, M. Marenco, F. Valvo, R. Orecchia

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: Glioblastoma is the most malignant and widespread brain tumor in adults, with a rapid clinical course. Recently, it has been hypothesized that L-DOPA plays a role in the diagnosis and treatment of glioblastoma. The aim of this study was to assess the effects of pretreatment with L-DOPA on the biological behavior of human T98G cells in vitro. Materials and Methods: T98G cells were treated with 50 μg/ml or 100 μg/ml of L-DOPA for 4 h and their morphology, growth rate, clonogenic survival and migratory capacity in basal conditions and after carbon ion irradiation were evaluated using standard methods. Results: Treated cells showed a lower growth rate and an increased migratory capacity that correlated with the dose of tested L-DOPA . Treatment with L-DOPA increased the growth rate of carbon ion irradiated T98G cells compared to control non-treated cells exposed to the same radiation dose. Conclusion: Our results open further questions about the overall advantage of L-DOPA treatment of glioblastoma. © 2019 International Institute of Anticancer Research. All Rights Reserved.
Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalAnticancer Res.
Volume39
Issue number1
DOIs
Publication statusPublished - 2019

Keywords

  • 18 F-DOPA
  • Carbon ion radiation
  • Glioblastoma
  • L-DOPA
  • Migration
  • Proliferation
  • levodopa
  • Article
  • cell kinetics
  • cell migration
  • cell structure
  • cell survival
  • clinical feature
  • clonogenesis
  • controlled study
  • drug effect
  • growth rate
  • human
  • human cell
  • in vitro study
  • ion therapy
  • priority journal
  • radiation dose
  • radiation response
  • T98G cell line
  • adult
  • cell motion
  • cell proliferation
  • glioblastoma
  • kinetics
  • pathology
  • tumor cell line
  • Adult
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Cell Survival
  • Dose-Response Relationship, Radiation
  • Heavy Ion Radiotherapy
  • Humans
  • Kinetics
  • Levodopa

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