Effects of magnesium sulphate on leptin-dependent platelet aggregation: An ex vivo study

A. Corsonello, A. Malara, D. De Domenico, M. C. Damiano, S. Mirone, S. Loddo, R. Ientile, F. Corica

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Magnesium sulphate has well known antiplatelet properties. Its effect on leptin-dependent platelet aggregation has not been studied previously. Thus, we performed this ex vivo study to investigate whether magnesium sulphate is able to inhibit leptin-dependent aggregation of human platelets. We obtained platelet rich plasma (PRP) from venous blood samples of 16 healthy male volunteers, and we measured ADP-induced platelet aggregation in the presence of leptin alone (5-500 ng/mL) or leptin and magnesium sulphate (0.25-8 mM). Platelet pre-incubation with leptin led to a significant and dose-dependent increase in ADP-induced platelet aggregation. Magnesium sulphate was able to inhibit the pro-aggregating effect of leptin in a dose-dependent manner. The inhibitory effect was apparent at 1 mM of magnesium sulphate concentration (% maximal aggregation=38.1±12.2) and reached its maximum at 8 mM (% maximal aggregation=20.0±7.8). Our results demonstrate that leptin-dependent platelet aggregation is inhibited by magnesium sulphate in a dose-dependent manner. It seems conceivable that the blocking of hydrolysis of phosphoinositide and of intracellular calcium mobilization by magnesium sulphate may be involved in these findings.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalMagnesium Research
Volume18
Issue number1
Publication statusPublished - Mar 2005

Fingerprint

Magnesium Sulfate
Platelets
Leptin
Platelet Aggregation
Agglomeration
Adenosine Diphosphate
Platelet-Rich Plasma
Phosphatidylinositols
Hydrolysis
Healthy Volunteers
Blood
Blood Platelets
Calcium
Plasmas

Keywords

  • Leptin
  • Magnesium sulphate
  • Platelet aggregation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Corsonello, A., Malara, A., De Domenico, D., Damiano, M. C., Mirone, S., Loddo, S., ... Corica, F. (2005). Effects of magnesium sulphate on leptin-dependent platelet aggregation: An ex vivo study. Magnesium Research, 18(1), 7-11.

Effects of magnesium sulphate on leptin-dependent platelet aggregation : An ex vivo study. / Corsonello, A.; Malara, A.; De Domenico, D.; Damiano, M. C.; Mirone, S.; Loddo, S.; Ientile, R.; Corica, F.

In: Magnesium Research, Vol. 18, No. 1, 03.2005, p. 7-11.

Research output: Contribution to journalArticle

Corsonello, A, Malara, A, De Domenico, D, Damiano, MC, Mirone, S, Loddo, S, Ientile, R & Corica, F 2005, 'Effects of magnesium sulphate on leptin-dependent platelet aggregation: An ex vivo study', Magnesium Research, vol. 18, no. 1, pp. 7-11.
Corsonello A, Malara A, De Domenico D, Damiano MC, Mirone S, Loddo S et al. Effects of magnesium sulphate on leptin-dependent platelet aggregation: An ex vivo study. Magnesium Research. 2005 Mar;18(1):7-11.
Corsonello, A. ; Malara, A. ; De Domenico, D. ; Damiano, M. C. ; Mirone, S. ; Loddo, S. ; Ientile, R. ; Corica, F. / Effects of magnesium sulphate on leptin-dependent platelet aggregation : An ex vivo study. In: Magnesium Research. 2005 ; Vol. 18, No. 1. pp. 7-11.
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