Abstract
Magnesium sulphate has well known antiplatelet properties. Its effect on leptin-dependent platelet aggregation has not been studied previously. Thus, we performed this ex vivo study to investigate whether magnesium sulphate is able to inhibit leptin-dependent aggregation of human platelets. We obtained platelet rich plasma (PRP) from venous blood samples of 16 healthy male volunteers, and we measured ADP-induced platelet aggregation in the presence of leptin alone (5-500 ng/mL) or leptin and magnesium sulphate (0.25-8 mM). Platelet pre-incubation with leptin led to a significant and dose-dependent increase in ADP-induced platelet aggregation. Magnesium sulphate was able to inhibit the pro-aggregating effect of leptin in a dose-dependent manner. The inhibitory effect was apparent at 1 mM of magnesium sulphate concentration (% maximal aggregation=38.1±12.2) and reached its maximum at 8 mM (% maximal aggregation=20.0±7.8). Our results demonstrate that leptin-dependent platelet aggregation is inhibited by magnesium sulphate in a dose-dependent manner. It seems conceivable that the blocking of hydrolysis of phosphoinositide and of intracellular calcium mobilization by magnesium sulphate may be involved in these findings.
| Original language | English |
|---|---|
| Pages (from-to) | 7-11 |
| Number of pages | 5 |
| Journal | Magnesium Research |
| Volume | 18 |
| Issue number | 1 |
| Publication status | Published - Mar 2005 |
Fingerprint
Keywords
- Leptin
- Magnesium sulphate
- Platelet aggregation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Effects of magnesium sulphate on leptin-dependent platelet aggregation : An ex vivo study. / Corsonello, A.; Malara, A.; De Domenico, D.; Damiano, M. C.; Mirone, S.; Loddo, S.; Ientile, R.; Corica, F.
In: Magnesium Research, Vol. 18, No. 1, 03.2005, p. 7-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of magnesium sulphate on leptin-dependent platelet aggregation
T2 - An ex vivo study
AU - Corsonello, A.
AU - Malara, A.
AU - De Domenico, D.
AU - Damiano, M. C.
AU - Mirone, S.
AU - Loddo, S.
AU - Ientile, R.
AU - Corica, F.
PY - 2005/3
Y1 - 2005/3
N2 - Magnesium sulphate has well known antiplatelet properties. Its effect on leptin-dependent platelet aggregation has not been studied previously. Thus, we performed this ex vivo study to investigate whether magnesium sulphate is able to inhibit leptin-dependent aggregation of human platelets. We obtained platelet rich plasma (PRP) from venous blood samples of 16 healthy male volunteers, and we measured ADP-induced platelet aggregation in the presence of leptin alone (5-500 ng/mL) or leptin and magnesium sulphate (0.25-8 mM). Platelet pre-incubation with leptin led to a significant and dose-dependent increase in ADP-induced platelet aggregation. Magnesium sulphate was able to inhibit the pro-aggregating effect of leptin in a dose-dependent manner. The inhibitory effect was apparent at 1 mM of magnesium sulphate concentration (% maximal aggregation=38.1±12.2) and reached its maximum at 8 mM (% maximal aggregation=20.0±7.8). Our results demonstrate that leptin-dependent platelet aggregation is inhibited by magnesium sulphate in a dose-dependent manner. It seems conceivable that the blocking of hydrolysis of phosphoinositide and of intracellular calcium mobilization by magnesium sulphate may be involved in these findings.
AB - Magnesium sulphate has well known antiplatelet properties. Its effect on leptin-dependent platelet aggregation has not been studied previously. Thus, we performed this ex vivo study to investigate whether magnesium sulphate is able to inhibit leptin-dependent aggregation of human platelets. We obtained platelet rich plasma (PRP) from venous blood samples of 16 healthy male volunteers, and we measured ADP-induced platelet aggregation in the presence of leptin alone (5-500 ng/mL) or leptin and magnesium sulphate (0.25-8 mM). Platelet pre-incubation with leptin led to a significant and dose-dependent increase in ADP-induced platelet aggregation. Magnesium sulphate was able to inhibit the pro-aggregating effect of leptin in a dose-dependent manner. The inhibitory effect was apparent at 1 mM of magnesium sulphate concentration (% maximal aggregation=38.1±12.2) and reached its maximum at 8 mM (% maximal aggregation=20.0±7.8). Our results demonstrate that leptin-dependent platelet aggregation is inhibited by magnesium sulphate in a dose-dependent manner. It seems conceivable that the blocking of hydrolysis of phosphoinositide and of intracellular calcium mobilization by magnesium sulphate may be involved in these findings.
KW - Leptin
KW - Magnesium sulphate
KW - Platelet aggregation
UR - http://www.scopus.com/inward/record.url?scp=18844365857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18844365857&partnerID=8YFLogxK
M3 - Article
C2 - 15945611
AN - SCOPUS:18844365857
VL - 18
SP - 7
EP - 11
JO - Magnesium Research
JF - Magnesium Research
SN - 0953-1424
IS - 1
ER -