Effects of magnesium sulphate on leptin-dependent platelet aggregation: An ex vivo study

A. Corsonello, A. Malara, D. De Domenico, M. C. Damiano, S. Mirone, S. Loddo, R. Ientile, F. Corica

Research output: Contribution to journalArticlepeer-review

Abstract

Magnesium sulphate has well known antiplatelet properties. Its effect on leptin-dependent platelet aggregation has not been studied previously. Thus, we performed this ex vivo study to investigate whether magnesium sulphate is able to inhibit leptin-dependent aggregation of human platelets. We obtained platelet rich plasma (PRP) from venous blood samples of 16 healthy male volunteers, and we measured ADP-induced platelet aggregation in the presence of leptin alone (5-500 ng/mL) or leptin and magnesium sulphate (0.25-8 mM). Platelet pre-incubation with leptin led to a significant and dose-dependent increase in ADP-induced platelet aggregation. Magnesium sulphate was able to inhibit the pro-aggregating effect of leptin in a dose-dependent manner. The inhibitory effect was apparent at 1 mM of magnesium sulphate concentration (% maximal aggregation=38.1±12.2) and reached its maximum at 8 mM (% maximal aggregation=20.0±7.8). Our results demonstrate that leptin-dependent platelet aggregation is inhibited by magnesium sulphate in a dose-dependent manner. It seems conceivable that the blocking of hydrolysis of phosphoinositide and of intracellular calcium mobilization by magnesium sulphate may be involved in these findings.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalMagnesium Research
Volume18
Issue number1
Publication statusPublished - Mar 2005

Keywords

  • Leptin
  • Magnesium sulphate
  • Platelet aggregation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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