Effects of mechanical ventilation at low lung volume on respiratory mechanics and nitric oxide exhalation in normal rabbits

Edgardo D'Angelo, Matteo Pecchiari, Patrizia Della Valle, Antonia Koutsoukou, Joseph Milic-Emili

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Lung mechanics, exhaled NO (NOe), and TNF-α in serum and bronchoalveolar lavage fluid were assessed in eight closed and eight open chest, normal anesthetized rabbits undergoing prolonged (3-4 h) mechanical ventilation (MV) at low volume with physiological tidal volumes (10 ml/kg). Relative to initial MV on positive end-expiratory pressure (PEEP), MV at low volume increased lung quasi-static elastance (+267 and +281%), airway (+471 and +382%) and viscolelastic resistance (+480 and +294%), and decreased NOe (-42 and -25%) in closed and open chest rabbits, respectively. After restoration of PEEP, viscoelastic resistance returned to control, whereas airway resistance remained elevated (+120 and +31%) and NOe low (-25 and -20%) in both groups of rabbits. Elastance remained elevated (+23%) only in closed-chest animals, being associated with interstitial pulmonary edema, as reflected by increased lung wet-to-dry weight ratio with normal albumin concentration in bronchoalveolar lavage fluid. In contrast, in 16 additional closed- and open-chest rabbits, there were no changes of lung mechanics or NOe after prolonged MV on PEEP only. At the end of prolonged MV, TNF-α was practically undetectable in serum, whereas its concentration in bronchoalveolar lavage fluid was low and similar in animals subjected or not subjected to ventilation at low volume (62 vs. 43 pg/ml). These results indicate that mechanical injury of peripheral airways due to their cyclic opening and closing during ventilation at low volume results in changes in lung mechanics and reduction in NOe and that these alterations are not mediated by a proinflammatory process, since this is expressed by TNF-α levels.

Original languageEnglish
Pages (from-to)433-444
Number of pages12
JournalJournal of Applied Physiology
Volume99
Issue number2
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Exhalation
Respiratory Mechanics
Artificial Respiration
Nitric Oxide
Positive-Pressure Respiration
Rabbits
Bronchoalveolar Lavage Fluid
Thorax
Lung
Mechanics
Pulmonary Edema
Ventilation
Airway Resistance
Tidal Volume
Serum
Albumins
Weights and Measures
Wounds and Injuries

Keywords

  • Exhaled vapor condensate
  • Interrupter resistance
  • Lung elastance
  • Proinflammatory cytokines
  • Viscoelasticity

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of mechanical ventilation at low lung volume on respiratory mechanics and nitric oxide exhalation in normal rabbits. / D'Angelo, Edgardo; Pecchiari, Matteo; Della Valle, Patrizia; Koutsoukou, Antonia; Milic-Emili, Joseph.

In: Journal of Applied Physiology, Vol. 99, No. 2, 08.2005, p. 433-444.

Research output: Contribution to journalArticle

D'Angelo, Edgardo ; Pecchiari, Matteo ; Della Valle, Patrizia ; Koutsoukou, Antonia ; Milic-Emili, Joseph. / Effects of mechanical ventilation at low lung volume on respiratory mechanics and nitric oxide exhalation in normal rabbits. In: Journal of Applied Physiology. 2005 ; Vol. 99, No. 2. pp. 433-444.
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