Effects of melatonin and pycnogenol on small artery structure and function in spontaneously hypertensive rats

Rita Rezzani, Enzo Porteri, Carolina De Ciuceis, Francesca Bonomini, Luigi F. Rodella, Silvia Paiardi, Gianluca E M Boari, Caterina Platto, Annamaria Pilu, Daniele Avanzi, Damiano Rizzoni, Enrico Agabiti Rosei

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Abstract

It was suggested that oxidative stress has a key role in the development of endothelial dysfunction, as well as microvascular structural alterations. Therefore, we have investigated 2 substances with antioxidant properties: melatonin and Pycnogenol. We treated 7 spontaneously hypertensive rats (SHRs) with melatonin and 7 with Pycnogenol for 6 weeks. We compared results obtained with those observed in 7 SHRs and 7 Wistar-Kyoto normotensive control rats kept untreated. Mesenteric small resistance arteries were dissected and mounted on a wire myograph, and a concentration-response curve to acetylcholine was performed. Aortic contents of metalloproteinase 2, Bax, inducible NO synthase, and cyclooxygenase 2 were evaluated, together with the aortic content of total collagen and collagen subtypes and apoptosis rate. A small reduction in systolic blood pressure was observed. A significant improvement in mesenteric small resistance artery structure and endothelial function was observed in rats treated with Pycnogenol and melatonin. Total aortic collagen content was significantly greater in untreated SHRs compared with Wistar-Kyoto control rats, whereas a full normalization was observed in treated rats. Apoptosis rate was increased in the aortas of untreated SHRs compared with Wistar-Kyoto control rats; an even more pronounced increase was observed in treated rats. Bax and metalloproteinase 2 expressions changed accordingly. Cyclooxygenase 2 and inducible NO synthase were more expressed in the aortas of untreated SHRs compared with Wistar-Kyoto control rats; this pattern was normalized by both treatments. In conclusion, our data suggest that treatment with Pycnogenol and melatonin may protect the vasculature, partly independent of blood pressure reduction, probably through their antioxidant effects.

Original languageEnglish
Pages (from-to)1373-1380
Number of pages8
JournalHypertension
Volume55
Issue number6
DOIs
Publication statusPublished - Jun 2010

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Inbred SHR Rats
Melatonin
Arteries
Inbred WKY Rats
Collagen
Metalloproteases
Cyclooxygenase 2
Blood Pressure
Nitric Oxide Synthase
Aorta
Antioxidants
Apoptosis
Acetylcholine
Oxidative Stress
pycnogenols

Keywords

  • Endothelial function
  • Melatonin
  • Pycnogenol oxidative stress

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Rezzani, R., Porteri, E., De Ciuceis, C., Bonomini, F., Rodella, L. F., Paiardi, S., ... Agabiti Rosei, E. (2010). Effects of melatonin and pycnogenol on small artery structure and function in spontaneously hypertensive rats. Hypertension, 55(6), 1373-1380. https://doi.org/10.1161/HYPERTENSIONAHA.109.148254

Effects of melatonin and pycnogenol on small artery structure and function in spontaneously hypertensive rats. / Rezzani, Rita; Porteri, Enzo; De Ciuceis, Carolina; Bonomini, Francesca; Rodella, Luigi F.; Paiardi, Silvia; Boari, Gianluca E M; Platto, Caterina; Pilu, Annamaria; Avanzi, Daniele; Rizzoni, Damiano; Agabiti Rosei, Enrico.

In: Hypertension, Vol. 55, No. 6, 06.2010, p. 1373-1380.

Research output: Contribution to journalArticle

Rezzani, R, Porteri, E, De Ciuceis, C, Bonomini, F, Rodella, LF, Paiardi, S, Boari, GEM, Platto, C, Pilu, A, Avanzi, D, Rizzoni, D & Agabiti Rosei, E 2010, 'Effects of melatonin and pycnogenol on small artery structure and function in spontaneously hypertensive rats', Hypertension, vol. 55, no. 6, pp. 1373-1380. https://doi.org/10.1161/HYPERTENSIONAHA.109.148254
Rezzani, Rita ; Porteri, Enzo ; De Ciuceis, Carolina ; Bonomini, Francesca ; Rodella, Luigi F. ; Paiardi, Silvia ; Boari, Gianluca E M ; Platto, Caterina ; Pilu, Annamaria ; Avanzi, Daniele ; Rizzoni, Damiano ; Agabiti Rosei, Enrico. / Effects of melatonin and pycnogenol on small artery structure and function in spontaneously hypertensive rats. In: Hypertension. 2010 ; Vol. 55, No. 6. pp. 1373-1380.
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