Effects of metabolic genotypes on intermediary biomarkers in subjects exposed to PAHS: Results from the EXPAH study

Seymour Garte, Emanuela Taioli, Sara Raimondi, Valentina Paracchini, Blanka Binkova, Radim J. Sram, Ivan Kalina, Todor A. Popov, Rajinder Singh, Peter B. Farmer

Research output: Contribution to journalArticle

Abstract

Data from the EXPAH project on PAH exposure and intermediary biomarkers were analyzed with respect to individual genotypes at seven metabolic gene loci. The GSTM1 null allele was associated with significantly higher levels of two biomarkers, malondialdehyde-2′-deoxyguanosine and benzo[a]pyrene DNA adducts in the total population from three Central and Eastern European countries. The CYP1B1 Leu/Val variant demonstrated effects on both markers of oxidative DNA damage in opposite directions, producing a higher level of M1dG with a trend from wild type (Leu/Leu) to heterozygotes to homozygous (Val/Val) variants, whereas the effects of these variants were reversed for 8-oxodG. Cluster Analysis was used to group composite genotypes in order to determine if combined genotypes of multiple loci could explain some of the variation seen with the biomarkers, expressed per unit of exposure, referred to as a sensitivity index. This analysis revealed two closely related genotypes each involving four of the loci (GSTM1*0/*0, CYP1A1*1*1, CYP1B1*1/*2, GSTP1*1/*1 and GSTT1*0/*0, CYP1A1*1*1, CYP1B1*1/*2, GSTP1*1/*1.) that conferred significant resistance to the DNA damaging effects of benzo[a]pyrene, measured as the level of a benzo[a]pyrene-like adduct per unit of benzo[a]pyrene exposed.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume620
Issue number1-2
DOIs
Publication statusPublished - Jul 1 2007

Keywords

  • Benzo[a]pyrene
  • Carcinogenic polycyclic aromatic hydrocarbons
  • DNA adducts
  • Metabolic genotype
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

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