Abstract
In this study twenty-two patients who had revision surgery for aseptic loosening of joint prostheses were examined. The concentration in serum of soluble products of corrosion from the implant, that is, chromium (Cr), cobalt (Co), and nickel (Ni) ions, and the number of white blood cells (leucocytes, myeloid cells, lymphocyte subpopulations) were measured. Twenty patients with no implants were used as controls. The patients who had revision surgery showed normal Ni concentration whereas by statistical analysis that same patient group was shown to have serum Cr and Co levels significantly higher than those of the control. By flow cytometry, a significant decrease of leucocytes, myeloid cells, lymphocytes, and CD16 populations as found in patients versus controls whereas CD3, CD4, CD8, and CD20 positive cells were decreased, but not significantly. In the arthroplasty patients the Cr concentrations were inversely correlated with some of the immunologic parameters while no significant correlation was found between Co levels and decreased lymphocyte subpopulations. Only in revision surgery patients with high Cr concentrations did we find a significant decrease of lymphocytes, namely of CD4 and CD16 positive cells; revision surgery patients with normal Cr concentrations did not show a significant decrease of lymphocyte subpopulations. These data suggest that the presence of metal ions, especially chromium, released from prosthesis components could be associated with changes of lymphocyte subpopulations in patients with loosening of joint prostheses.
| Original language | English |
|---|---|
| Pages (from-to) | 543-550 |
| Number of pages | 8 |
| Journal | Journal of Biomedical Materials Research |
| Volume | 47 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 |
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Keywords
- Implant
- Loosening
- Metal ions
- White blood cells
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
Effects of metal ions on white blood cells of patients with failed total joint arthroplasties. / Savarino, L.; Granchi, D.; Ciapetti, G.; Stea, S.; Donati, M. E.; Zinghi, G.; Fontanesi, G.; Rotini, R.; Montanaro, L.
In: Journal of Biomedical Materials Research, Vol. 47, No. 4, 1999, p. 543-550.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of metal ions on white blood cells of patients with failed total joint arthroplasties
AU - Savarino, L.
AU - Granchi, D.
AU - Ciapetti, G.
AU - Stea, S.
AU - Donati, M. E.
AU - Zinghi, G.
AU - Fontanesi, G.
AU - Rotini, R.
AU - Montanaro, L.
PY - 1999
Y1 - 1999
N2 - In this study twenty-two patients who had revision surgery for aseptic loosening of joint prostheses were examined. The concentration in serum of soluble products of corrosion from the implant, that is, chromium (Cr), cobalt (Co), and nickel (Ni) ions, and the number of white blood cells (leucocytes, myeloid cells, lymphocyte subpopulations) were measured. Twenty patients with no implants were used as controls. The patients who had revision surgery showed normal Ni concentration whereas by statistical analysis that same patient group was shown to have serum Cr and Co levels significantly higher than those of the control. By flow cytometry, a significant decrease of leucocytes, myeloid cells, lymphocytes, and CD16 populations as found in patients versus controls whereas CD3, CD4, CD8, and CD20 positive cells were decreased, but not significantly. In the arthroplasty patients the Cr concentrations were inversely correlated with some of the immunologic parameters while no significant correlation was found between Co levels and decreased lymphocyte subpopulations. Only in revision surgery patients with high Cr concentrations did we find a significant decrease of lymphocytes, namely of CD4 and CD16 positive cells; revision surgery patients with normal Cr concentrations did not show a significant decrease of lymphocyte subpopulations. These data suggest that the presence of metal ions, especially chromium, released from prosthesis components could be associated with changes of lymphocyte subpopulations in patients with loosening of joint prostheses.
AB - In this study twenty-two patients who had revision surgery for aseptic loosening of joint prostheses were examined. The concentration in serum of soluble products of corrosion from the implant, that is, chromium (Cr), cobalt (Co), and nickel (Ni) ions, and the number of white blood cells (leucocytes, myeloid cells, lymphocyte subpopulations) were measured. Twenty patients with no implants were used as controls. The patients who had revision surgery showed normal Ni concentration whereas by statistical analysis that same patient group was shown to have serum Cr and Co levels significantly higher than those of the control. By flow cytometry, a significant decrease of leucocytes, myeloid cells, lymphocytes, and CD16 populations as found in patients versus controls whereas CD3, CD4, CD8, and CD20 positive cells were decreased, but not significantly. In the arthroplasty patients the Cr concentrations were inversely correlated with some of the immunologic parameters while no significant correlation was found between Co levels and decreased lymphocyte subpopulations. Only in revision surgery patients with high Cr concentrations did we find a significant decrease of lymphocytes, namely of CD4 and CD16 positive cells; revision surgery patients with normal Cr concentrations did not show a significant decrease of lymphocyte subpopulations. These data suggest that the presence of metal ions, especially chromium, released from prosthesis components could be associated with changes of lymphocyte subpopulations in patients with loosening of joint prostheses.
KW - Implant
KW - Loosening
KW - Metal ions
KW - White blood cells
UR - http://www.scopus.com/inward/record.url?scp=0031573493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031573493&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4636(19991215)47:4<543::AID-JBM11>3.0.CO;2-G
DO - 10.1002/(SICI)1097-4636(19991215)47:4<543::AID-JBM11>3.0.CO;2-G
M3 - Article
C2 - 10497290
AN - SCOPUS:0031573493
VL - 47
SP - 543
EP - 550
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 4
ER -