Effects of metformin with or without supplementation with folate on homocysteine levels and vascular endothelium of women with polycystic ovary syndrome

Stefano Palomba, Angela Falbo, Francesco Giallauria, Tiziana Russo, Achille Tolino, Fulvio Zullo, Annamaria Colao, Francesco Orio

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

OBJECTIVE - To evaluate whether the administration of metformin exerts any effects on serum homocysteine (Hcy) levels in patients with polycystic ovary syndrome (PCOS) and whether supplementation with folate enhances the positive effects of metformin on the structure and function of the vascular endothelium. RESEARCH DESIGN AND METHODS - A total of 50 patients affected by PCOS, without additional metabolic or cardiovascular diseases, were enrolled in a prospective nonrandomized placebo-controlled double-blind clinical study. They were grouped into two treatment arms that were matched for age and BMI. Patients were treated with a 6-month course of metformin (1,700 mg daily) plus folic acid (400 μg daily; experimental group, n = 25) or placebo (control group, n = 25). Complete hormonal and metabolic patterns, serum Hcy, folate, vitamin B12, endothelin-1 levels, brachial artery diameter at the baseline (BAD-B) and after reactive hyperemia (BAD-RH), flow-mediated dilation, and intima-media thickness in both common carotid arteries were evaluated. RESULTS - After treatment, a significant increase in serum Hcy levels was observed in the control group compared with the baseline values and the experimental group. A beneficial effect was observed in the concentrations of BAD-B, BAD-RH, flow-mediated dilation, intima-media thickness, and serum endothelin-1 in both groups. However, the results were improved more significantly in the experimental group than in the control subjects. CONCLUSIONS - Metformin exerts a slight but significant deleterious effect on serum Hcy levels in patients with PCOS, and supplementation with folate is useful to increase the beneficial effect of metformin on the vascular endothelium.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalDiabetes Care
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 2010

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Polycystic Ovary Syndrome
Metformin
Vascular Endothelium
Homocysteine
Folic Acid
Serum
Brachial Artery
Hyperemia
Endothelin-1
Control Groups
Dilatation
Placebos
Common Carotid Artery
Metabolic Diseases
Vitamin B 12
Double-Blind Method
Research Design
Cardiovascular Diseases
Therapeutics

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialised Nursing

Cite this

Effects of metformin with or without supplementation with folate on homocysteine levels and vascular endothelium of women with polycystic ovary syndrome. / Palomba, Stefano; Falbo, Angela; Giallauria, Francesco; Russo, Tiziana; Tolino, Achille; Zullo, Fulvio; Colao, Annamaria; Orio, Francesco.

In: Diabetes Care, Vol. 33, No. 2, 02.2010, p. 246-251.

Research output: Contribution to journalArticle

Palomba, Stefano ; Falbo, Angela ; Giallauria, Francesco ; Russo, Tiziana ; Tolino, Achille ; Zullo, Fulvio ; Colao, Annamaria ; Orio, Francesco. / Effects of metformin with or without supplementation with folate on homocysteine levels and vascular endothelium of women with polycystic ovary syndrome. In: Diabetes Care. 2010 ; Vol. 33, No. 2. pp. 246-251.
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AU - Falbo, Angela

AU - Giallauria, Francesco

AU - Russo, Tiziana

AU - Tolino, Achille

AU - Zullo, Fulvio

AU - Colao, Annamaria

AU - Orio, Francesco

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N2 - OBJECTIVE - To evaluate whether the administration of metformin exerts any effects on serum homocysteine (Hcy) levels in patients with polycystic ovary syndrome (PCOS) and whether supplementation with folate enhances the positive effects of metformin on the structure and function of the vascular endothelium. RESEARCH DESIGN AND METHODS - A total of 50 patients affected by PCOS, without additional metabolic or cardiovascular diseases, were enrolled in a prospective nonrandomized placebo-controlled double-blind clinical study. They were grouped into two treatment arms that were matched for age and BMI. Patients were treated with a 6-month course of metformin (1,700 mg daily) plus folic acid (400 μg daily; experimental group, n = 25) or placebo (control group, n = 25). Complete hormonal and metabolic patterns, serum Hcy, folate, vitamin B12, endothelin-1 levels, brachial artery diameter at the baseline (BAD-B) and after reactive hyperemia (BAD-RH), flow-mediated dilation, and intima-media thickness in both common carotid arteries were evaluated. RESULTS - After treatment, a significant increase in serum Hcy levels was observed in the control group compared with the baseline values and the experimental group. A beneficial effect was observed in the concentrations of BAD-B, BAD-RH, flow-mediated dilation, intima-media thickness, and serum endothelin-1 in both groups. However, the results were improved more significantly in the experimental group than in the control subjects. CONCLUSIONS - Metformin exerts a slight but significant deleterious effect on serum Hcy levels in patients with PCOS, and supplementation with folate is useful to increase the beneficial effect of metformin on the vascular endothelium.

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