Effects of microvascular clamp duration on the wall of rat femoral artery

Luigi Valdatta, T. Congiu, M. Buoro, A. Thione, A. Faga, M. Reguzzoni

Research output: Contribution to journalArticlepeer-review

Abstract

We performed a morphological study to establish if and to what extent increasing microvascular clamp duration affects the arterial wall. 15 Sprague-Dawley male rats were divided in 3 groups (A, B, C); the right femoral artery of each animal was clamped for 10 minutes (group A), 30 minutes (group B) and 60 minutes (group C) with a double microvascular clamp for arteries of the Kleinert-Kutz model (closing pressure of 25-30 g/mm2). The left femoral artery was left untouched in-each animal as control. 7 days after the first operation right and left femoral arteries were explanted and specimens were prepared for optic microscope (OM) and transmission electronic microscope (TEM) observation. The morphological analysis showed that using microvascular clamps that produce a costant pressure, even for prolonged clamp duration, no wall damage could be detected as an interruption of the endothelial layer, nor as a disorganization of smooth muscular cells structure. Viceversa OM and TEM observation showed the presence by the clamped points of an inflammatory infiltration increased for longer clamp duration. These events could reasonably generate vascuar stenosis which could reduce blood flow through the anastomosis, the same, if not in greater proportion, can reasonably be thought to happen in human small arteries.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalRivista Italiana di Chirurgia Plastica
Volume33
Issue number1-2
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Surgery

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