Effects of modifications near the 2-, 3- and 4-fold symmetry axes on human ferritin renaturation

P. Santambrogio, P. Pinto, S. Levi, A. Cozzi, E. Rovida, Alberto Albertini, Peter Artymiuk, Pauline M. Harrison, Paolo Arosio

Research output: Contribution to journalArticlepeer-review

Abstract

Ferritin is a protein of 24 subunits which assemble into a shell with 432 point symmetry. It can be denatured reversibly in acidic guanidine hydrochloride, with the formation of poorly populated renaturation intermediates. In order to increase the accumulation of intermediates and to study the mechanism of ferritin renaturation, we analysed variants of the human ferritin H-chain altered at the N-terminus Δ1-13), near the 4-fold axis (Leu-169 → Arg), the 3-fold axis (Asp-131 → Ile + Glu-134 → Phe) or the 2-fold axis (Ile-85 → Cys). We also carried out specific chemical modifications of Cys-130 (near the 3-fold axis) and Cys-85 (near the 2-fold axis). Renaturation of the modified ferritins yielded assembly intermediates that differed in size and physical properties. Alterations of residues around the 2-, 4- and 3-fold axes produced subunit monomers, dimers and higher oligomers respectively. All these intermediates could be induced to assemble into ferritin 24-mers by concentrating them or by co-renaturing them with wild-type H-ferritin. The results support the hypothesis that the symmetric subunit dimers are the building blocks of ferritin assembly, and are consistent with a reassembly pathway involving the coalescence of dimers, probably around the 4-fold axis, followed by stepwise addition of dimers until the 24-mer cage is completed. In addition they show that assembly interactions are responsible for the large hysteresis of folding and unfolding plots. The implications of the studies for in vivo heteropolymer formation in vertebrates, which have two types of ferritin chain (H and L), are discussed.

Original languageEnglish
Pages (from-to)461-468
Number of pages8
JournalBiochemical Journal
Volume322
Issue number2
Publication statusPublished - Mar 1 1997

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Effects of modifications near the 2-, 3- and 4-fold symmetry axes on human ferritin renaturation'. Together they form a unique fingerprint.

Cite this