Effects of palladium nanoparticles on the cytokine release from peripheral blood mononuclear cells of palladium-sensitized women

Marcella Reale, Giovina Vianale, Lavinia Vittoria Lotti, Renato Mariani-Costantini, Silvia Perconti, Antonio Cristaudo, Kerstin Leopold, Andrea Antonucci, Luca Di Giampaolo, Ivo Iavicoli, Mario Di Gioacchino, Paolo Boscolo

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Abstract

Objective: To investigate the effects of palladium (Pd) nanoparticles on cytokine release from peripheral blood mononuclear cells (PBMCs) of control or Pd-sensitized nonatopic women. Methods: TNF-α, IL-5, IL-10 and IFN-γ release and/or expression from PBMCs incubated in presence of 5 to 10 nm Pd nanoparticles or Pd salt (potassium hexachloropalladate) were determined by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction analysis. Transmission electronmicroscopy was performed. Results: In lipopolysaccharide-stimulated PBMCs from controls, Pd salt inhibited IFN-γ and IL-10 release, whereas Pd nanoparticles enhanced IFN-γ release and inhibited TNF-α secretion. In lipopolysaccharide-stimulated PBMCs from Pd-sensitized women showing high IFN-γ release, Pd nanoparticles inhibited TNF-α release and Pd salt IL-10 release. TNF-α and IFN-γ release and messenger RNA expression were correlated. Transmission electronmicroscopy demonstrated uptake of nanoparticles in the endocytic compartment and activation of autophagy. Conclusions: Palladium ions and nanoparticles exert different effects in vitro on the expression and release of cytokines.

Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalJournal of Occupational and Environmental Medicine
Volume53
Issue number9
DOIs
Publication statusPublished - Sep 2011

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Reale, M., Vianale, G., Lotti, L. V., Mariani-Costantini, R., Perconti, S., Cristaudo, A., Leopold, K., Antonucci, A., Di Giampaolo, L., Iavicoli, I., Di Gioacchino, M., & Boscolo, P. (2011). Effects of palladium nanoparticles on the cytokine release from peripheral blood mononuclear cells of palladium-sensitized women. Journal of Occupational and Environmental Medicine, 53(9), 1054-1060. https://doi.org/10.1097/JOM.0b013e318228115e