TY - JOUR
T1 - Effects of palladium nanoparticles on the cytokine release from peripheral blood mononuclear cells of palladium-sensitized women
AU - Reale, Marcella
AU - Vianale, Giovina
AU - Lotti, Lavinia Vittoria
AU - Mariani-Costantini, Renato
AU - Perconti, Silvia
AU - Cristaudo, Antonio
AU - Leopold, Kerstin
AU - Antonucci, Andrea
AU - Di Giampaolo, Luca
AU - Iavicoli, Ivo
AU - Di Gioacchino, Mario
AU - Boscolo, Paolo
PY - 2011/9
Y1 - 2011/9
N2 - Objective: To investigate the effects of palladium (Pd) nanoparticles on cytokine release from peripheral blood mononuclear cells (PBMCs) of control or Pd-sensitized nonatopic women. Methods: TNF-α, IL-5, IL-10 and IFN-γ release and/or expression from PBMCs incubated in presence of 5 to 10 nm Pd nanoparticles or Pd salt (potassium hexachloropalladate) were determined by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction analysis. Transmission electronmicroscopy was performed. Results: In lipopolysaccharide-stimulated PBMCs from controls, Pd salt inhibited IFN-γ and IL-10 release, whereas Pd nanoparticles enhanced IFN-γ release and inhibited TNF-α secretion. In lipopolysaccharide-stimulated PBMCs from Pd-sensitized women showing high IFN-γ release, Pd nanoparticles inhibited TNF-α release and Pd salt IL-10 release. TNF-α and IFN-γ release and messenger RNA expression were correlated. Transmission electronmicroscopy demonstrated uptake of nanoparticles in the endocytic compartment and activation of autophagy. Conclusions: Palladium ions and nanoparticles exert different effects in vitro on the expression and release of cytokines.
AB - Objective: To investigate the effects of palladium (Pd) nanoparticles on cytokine release from peripheral blood mononuclear cells (PBMCs) of control or Pd-sensitized nonatopic women. Methods: TNF-α, IL-5, IL-10 and IFN-γ release and/or expression from PBMCs incubated in presence of 5 to 10 nm Pd nanoparticles or Pd salt (potassium hexachloropalladate) were determined by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction analysis. Transmission electronmicroscopy was performed. Results: In lipopolysaccharide-stimulated PBMCs from controls, Pd salt inhibited IFN-γ and IL-10 release, whereas Pd nanoparticles enhanced IFN-γ release and inhibited TNF-α secretion. In lipopolysaccharide-stimulated PBMCs from Pd-sensitized women showing high IFN-γ release, Pd nanoparticles inhibited TNF-α release and Pd salt IL-10 release. TNF-α and IFN-γ release and messenger RNA expression were correlated. Transmission electronmicroscopy demonstrated uptake of nanoparticles in the endocytic compartment and activation of autophagy. Conclusions: Palladium ions and nanoparticles exert different effects in vitro on the expression and release of cytokines.
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U2 - 10.1097/JOM.0b013e318228115e
DO - 10.1097/JOM.0b013e318228115e
M3 - Article
C2 - 21866053
AN - SCOPUS:80052765046
VL - 53
SP - 1054
EP - 1060
JO - Journal of Occupational and Environmental Medicine
JF - Journal of Occupational and Environmental Medicine
SN - 1076-2752
IS - 9
ER -