Effects of peptidic fractions from Saccharomyces cerevisiae culture on growth and metabolism of the ruminal bacteria Megasphaera elsdenii

Filippo Rossi, Aldo Di Luccia, Donatella Vincenti, Pier Sandro Cocconcelli

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The aim of this study was to evaluate the effects of peptide fractions purified from a Saccharomyces cerevisiae product on the growth of the ruminal bacterium Megasphaera elsdenii. Such compounds have been shown to affect the growth and metabolism of M. elsdenii, the ruminal bacteria which uses lactate the most. A hydrophilic fraction purified by means of HPLC has proven to stimulate cell growth, the production of butyrate (+100%) and valerate (+76.1%) as well as the metabolization of lactate (+ 16.9%). This chromatographic fraction was further characterized and seven positively charged peptides were identified. It was observed that two peptides rich in lysine and histidine were the most effective in stimulating growth (+ 18.5%) and lactate utilization (+ 74.1%) in M. elsdenii. These results show that, in addition to previously studied factors such as the supply of nutrients or oxygen removal, the presence of peptidic fractions is involved in the stimulation of bacterial growth by S. cerevisiae.

Original languageEnglish
Pages (from-to)177-186
Number of pages10
JournalAnimal Research
Volume53
Issue number3
DOIs
Publication statusPublished - May 2004

Fingerprint

Megasphaera elsdenii
rumen bacteria
lactates
Saccharomyces cerevisiae
peptides
metabolism
butyrates
histidine
microbial growth
cell growth
lysine
oxygen
nutrients

Keywords

  • Megasphaera elsdenii
  • Peptides
  • Rumen
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of peptidic fractions from Saccharomyces cerevisiae culture on growth and metabolism of the ruminal bacteria Megasphaera elsdenii. / Rossi, Filippo; Di Luccia, Aldo; Vincenti, Donatella; Cocconcelli, Pier Sandro.

In: Animal Research, Vol. 53, No. 3, 05.2004, p. 177-186.

Research output: Contribution to journalArticle

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