Effects of perphenazine on the metabolism of inositol phospholipids in SK-N-BE(2) human neuroblastoma cells

Laura Pacini, Cristina Limatola, Eleonora Palma, Angelo Spinedi

Research output: Contribution to journalArticle

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Abstract

Administration of myo-[3H]inositol to SK-N-BE(2) human neuroblastoma cells for 24 hr resulted in equilibrium labelling of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2), as well as in retention of a large intracellular pool of free myo-[3H]inositol. Equilibrium labelling was no longer observed when cells were treated for 2 hr with 20 μM perphenazine (PPZ) in label-free medium; under these conditions, myo-[3H]inositol from the retained intracellular pool was incorporated into PI and PIP but not into PIP2. Analysis of water-soluble myo-[3H]inositol derivatives and inositol 1,4,5-trisphosphate mass determination indicated that PPZ did not stimulate phosphoinositide hydrolysis by phospholipase C. These results indicate that PPZ raises PI and PIP levels, whereas it is ineffective in expanding the PIP2 pool. The latter effect is not due to a concomitant synthesis and hydrolysis of this lipid.

Original languageEnglish
Pages (from-to)1655-1657
Number of pages3
JournalBiochemical Pharmacology
Volume48
Issue number8
DOIs
Publication statusPublished - Oct 18 1994

Fingerprint

Perphenazine
Inositol
Phosphatidylinositols
Neuroblastoma
Metabolism
Hydrolysis
Labeling
Phosphoinositide Phospholipase C
Inositol 1,4,5-Trisphosphate
Type C Phospholipases
Labels
Lipids
Water
Derivatives
phosphatidylinositol 4-phosphate

Keywords

  • inositol phospholipids
  • neuroblastoma
  • perphenazine

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of perphenazine on the metabolism of inositol phospholipids in SK-N-BE(2) human neuroblastoma cells. / Pacini, Laura; Limatola, Cristina; Palma, Eleonora; Spinedi, Angelo.

In: Biochemical Pharmacology, Vol. 48, No. 8, 18.10.1994, p. 1655-1657.

Research output: Contribution to journalArticle

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AB - Administration of myo-[3H]inositol to SK-N-BE(2) human neuroblastoma cells for 24 hr resulted in equilibrium labelling of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2), as well as in retention of a large intracellular pool of free myo-[3H]inositol. Equilibrium labelling was no longer observed when cells were treated for 2 hr with 20 μM perphenazine (PPZ) in label-free medium; under these conditions, myo-[3H]inositol from the retained intracellular pool was incorporated into PI and PIP but not into PIP2. Analysis of water-soluble myo-[3H]inositol derivatives and inositol 1,4,5-trisphosphate mass determination indicated that PPZ did not stimulate phosphoinositide hydrolysis by phospholipase C. These results indicate that PPZ raises PI and PIP levels, whereas it is ineffective in expanding the PIP2 pool. The latter effect is not due to a concomitant synthesis and hydrolysis of this lipid.

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