Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: An immunocytochemical study

Emilio Bajetta, Luigi Celio, Angelo Di Leo, Cesare Bartoli, Silvana Pilotti, Monica Leutner, Aldo Bono, Leonardo Ferrari, Roberto Buzzoni, Nicoletta Zilembo, Daniela De Candis, Daniele Moglia

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Abstract

The effects of the short-term pre-operative administration of tamoxifen (TAM, 20 mg once daily) on the tumor levels of steroid receptors and the nuclear proliferation Ki-67 antigen, were investigated in 32 elderly patients with hormone-sensitive, operable primary breast cancer by means of fine- needle aspiration biopsy (FNAB). The FNAB smears before (pre-TAM) and after six weeks of treatment (post-TAM) were stained immunocytochemically in order to obtain an H-score for steroid receptors, and the percentage of cellular nuclei containing Ki-67. The mean oestrogen receptor (ER) score between the pre- and post-TAM specimens fell from 181.2±9.7 (± SEM) to 148.1±7.9 (Wilcoxon's matched-pairs signed-rank test, p=0.01) and there was also a significant decrease in both the mean progesterone receptor (PgR) score (178.4±10.6 vs 148.5±10.6; p=0.01) and mean Ki-67 index (8.2%±1.2 vs 4.9%±0.9; p=0.0002). The reliability of FNAB as a sampling method was checked by comparing the results of the immunocytochemical assay (ICA) of the post-TAM biopsies with those of the immunohistochemical assay (IHA) of the corresponding excised tumors. There was a positive correlation between the ICA and IHA scores: ER (Spearman's correlation coefficient, rho=0.66, p

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalInternational Journal of Oncology
Volume12
Issue number4
Publication statusPublished - Apr 1998

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Keywords

  • Immunocytochemistry
  • Primary breast cancer
  • Steroid receptors
  • Tamoxifen
  • The Ki-67 antigen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bajetta, E., Celio, L., Di Leo, A., Bartoli, C., Pilotti, S., Leutner, M., Bono, A., Ferrari, L., Buzzoni, R., Zilembo, N., De Candis, D., & Moglia, D. (1998). Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: An immunocytochemical study. International Journal of Oncology, 12(4), 853-858.