Effects of sodium hypochlorite and ethylenediaminetetraacetic acid on rotary nickel-titanium instruments evaluated using atomic force microscopy

G. Ametrano, V. D'Antò, M. P. Di Caprio, M. Simeone, S. Rengo, G. Spagnuolo

Research output: Contribution to journalArticle

Abstract

Aim To use atomic force microscopy (AFM) to evaluate the effects of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA) on the surface characteristics of ProTaper rotary nickel-titanium instruments. Methodology A total of twenty ProTaper (Dentsply Maillefer, Ballaigues, Switzerland) instruments (S1, S2, F1, F2) were divided into five groups: no immersion, immersion in 5.25% NaOCl for 5 or 10min and immersion in 17% EDTA for 5 or 10min. Twenty surface areas along 3-mm sections at the tip of the files (perfect squares of 1×1μm) were analysed by AFM operating in contact mode under ambient conditions. Three-dimensional images (400×400 lines) were processed using Gwyddion software, and the roughness average (Ra) and the root mean square value (RMS) of the scanned surface profiles were recorded. Data were analysed by means of anova and paired samples t-test. Results Three-dimensional AFM images of the surface of ProTaper instruments, including new and those immersed in NaOCl and EDTA solutions, revealed topographic irregularities at the nanometric scale. RMS and Ra values of instruments treated with NaOCl and EDTA solutions were statistically higher than that of the new ones (P

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalInternational Endodontic Journal
Volume44
Issue number3
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Atomic force microscopy
  • EDTA
  • NaOCl
  • NiTi

ASJC Scopus subject areas

  • Dentistry(all)

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