Effects of specific humoral immunity on DNA-adduct formation in Swiss mice treated with benzo[a]pyrene

Rossella Galati, Guliana Falasca, Alessandra Verdina

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In order to study the effect of possible modulating factors on DNA-binding carcinogens, we investigated the role of specific immune response on racemic 7,t-8-dihydroxy-t-9,10- epoxy-7,8,9, 10-tetrahydro-benzo[a]pyrene ((±)-anti BPDE)-DNA (BPDE-DNA) adduct formation. Anti BPDE Immunoglobulin G (IgG) were elicited in Swiss mice before subsequent carcinogen administration. The immunization schedule consisted of four weekly subcutaneous injections of both (±)-anti BPDE-gelatin (BPDE-Gel) and DNA (BPDE-DNA) conjugate, followed by a final immunogen injection 14 days later. The immunization procedure resulted in the production of specific anti-BPDE antibodies in all treated animals. One week after the end of the immunization procedure, both groups of immunized and non immunized mice were treated with different doses of B[a]P (25-50-100-200 mg B[a]P/Kg body weight) by intraperitoneal injection. Seven days after treatment, the mice were sacrified. Adduct levels were detected by competitive ELISA by using optimal conditions established in our laboratory an highly specific and sensitive IgG anti BPDE-DNA induced in rabbit. The determination of DNA adducts in liver revealed significantly lower B[a]P adduct level in liver of immunized mice with respect to non-immunized animals. This result confirms those obtained for 2-acetylaminofluorene (2-AAF) in a previous work: the specific humoral immunity elicited by repeated carcinogen exposure may be able to modulate the genotoxic effect induced by subsequent carcinogen administration.

Original languageEnglish
Pages (from-to)2101-2104
Number of pages4
JournalAnticancer Research
Volume19
Issue number3 A
Publication statusPublished - 1999

Fingerprint

DNA Adducts
Benzo(a)pyrene
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Humoral Immunity
Carcinogens
Immunization
Immunoglobulin G
Immunization Schedule
2-Acetylaminofluorene
Liver
Subcutaneous Injections
Gelatin
Intraperitoneal Injections
Anti-Idiotypic Antibodies
Gels
Enzyme-Linked Immunosorbent Assay
Body Weight
Rabbits
Injections
DNA

Keywords

  • Benzo[a]pyrene
  • DNA adducts
  • ELISA
  • Humoral immunity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects of specific humoral immunity on DNA-adduct formation in Swiss mice treated with benzo[a]pyrene. / Galati, Rossella; Falasca, Guliana; Verdina, Alessandra.

In: Anticancer Research, Vol. 19, No. 3 A, 1999, p. 2101-2104.

Research output: Contribution to journalArticle

Galati, Rossella ; Falasca, Guliana ; Verdina, Alessandra. / Effects of specific humoral immunity on DNA-adduct formation in Swiss mice treated with benzo[a]pyrene. In: Anticancer Research. 1999 ; Vol. 19, No. 3 A. pp. 2101-2104.
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