Effects of surface compositional and structural heterogeneity on nanoparticle-protein interactions: Different protein configurations

Rixiang Huang, Randy P. Carney, Kaoru Ikuma, Francesco Stellacci, Boris L T Lau

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

As nanoparticles (NPs) enter into biological systems, they are immediately exposed to a variety and concentration of proteins. The physicochemical interactions between proteins and NPs are influenced by the surface properties of the NPs. To identify the effects of NP surface heterogeneity, the interactions between bovine serum albumin (BSA) and gold NPs (AuNPs) with similar chemical composition but different surface structures were investigated. Different interaction modes and BSA conformations were studied by dynamic light scattering, circular dichroism spectroscopy, fluorescence quenching and isothermal titration calorimetry (ITC). Depending on the surface structure of AuNPs, BSA seems to adopt either a side-on or an end-on conformation on AuNPs. ITC demonstrated that the adsorption of BSA onto AuNPs with randomly distributed polar and nonpolar groups was primarily driven by electrostatic interaction, and all BSA were adsorbed in the same process. The adsorption of BSA onto AuNPs covered with alternating domains of polar and nonpolar groups was a combination of different interactions. Overall, the results of this study point to the potential for utilizing nanoscale manipulation of NP surfaces to control the resulting NP-protein interactions.

Original languageEnglish
Pages (from-to)5402-5412
Number of pages11
JournalACS Nano
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 24 2014

Fingerprint

Nanoparticles
Bovine Serum Albumin
albumins
serums
proteins
Proteins
nanoparticles
configurations
interactions
Calorimetry
Titration
Surface structure
titration
Adsorption
Conformations
heat measurement
Circular dichroism spectroscopy
adsorption
Surface Properties
Fluorescence Spectrometry

Keywords

  • bionano interface
  • bovine serum albumin
  • gold nanoparticles
  • protein conformation
  • surface heterogeneity

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

Effects of surface compositional and structural heterogeneity on nanoparticle-protein interactions : Different protein configurations. / Huang, Rixiang; Carney, Randy P.; Ikuma, Kaoru; Stellacci, Francesco; Lau, Boris L T.

In: ACS Nano, Vol. 8, No. 6, 24.06.2014, p. 5402-5412.

Research output: Contribution to journalArticle

Huang, Rixiang ; Carney, Randy P. ; Ikuma, Kaoru ; Stellacci, Francesco ; Lau, Boris L T. / Effects of surface compositional and structural heterogeneity on nanoparticle-protein interactions : Different protein configurations. In: ACS Nano. 2014 ; Vol. 8, No. 6. pp. 5402-5412.
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