Effects of 'systemic' budesonide concentrations on in vitro allergen-induced activation of blood mononuclear cells isolated from asthmatic patients

S. Oddera, M. Silvestri, O. Sacco, S. Lantero, M. C. Morelli, G. A. Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

Blood levels of inhaled corticosteroids are significantly lower than those measured in the lung, but their concentration could still have anti-inflammatory effects. To determine whether budesonide, at concentrations similar to those obtained in blood after drug inhalation (10 -9 M), could downregulate the allergen-induced activation of mononuclear cells, we studied 21 atopic patients, sensitized to Dermatophagoides pteronyssinus (Der p). On blood mononuclear cells, isolated from these patients, incubated with Der p allergen extract and with or without budesonide, we evaluated: 1) the proliferative response of T cells; 2) the expression of two surface activation markers, the HLA-DR antigens and the interleukin (IL)-2 receptors; and 3) the release of cytokines known to modulate the allergic processes. Allergen-induced T-cell proliferation was associated with increased HLA-DR antigen and IL-2 receptor expression (P-9 and 10 -10 M). A significant inhibitory effect on T-cell proliferation was also present when budesonide (10 -9 M) was added to the cell cultures 3 or 5 days after the beginning of the cell cultures. In addition, budesonide 10 -9 M significantly decreased the expression of IL-2 receptors (P

Original languageEnglish
Pages (from-to)397-404
Number of pages8
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume50
Issue number5
Publication statusPublished - 1995

Keywords

  • Atopy
  • Corticosteroids
  • Dermatophagoides pteronyssinus
  • Granulocyte/macrophage colony-stimulating factor (GM-CSF)
  • HLA-DR
  • Interferon-gama
  • Interleukin-1β
  • Interleukin-2
  • Interleukin-2 receptor
  • Interleulin-4
  • Tumor necrosis factor-alpha

ASJC Scopus subject areas

  • Immunology

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