Effetti del β-blocco sui sistemi di transporto eritrocitario del Na +: Valutazione durante stimolo con cold pressor test in pazienti con ipertensione essenziale

Translated title of the contribution: Effects of the β-blockade on the systems of red blood cells sodium transport: Evaluation during stimulus with cold pressor test in patients with essential hypertension

Antonino Saitta, Michele N. Saitta, Michele Bonaiuto, Maria Castaldo, Adriana Sardo, Antonella Messina, Maurizio Cinquegrani, Giovanni Squadrito, Giuseppe M. Campo, Francesco Squadrito

Research output: Contribution to journalArticle

Abstract

Several studies have shown in essential hypertension alterations of the transmembrane red blood cells sodium fluxes, as an involvement, especially in the early phases, also of the adrenergic system. In this study we evaluated the behaviour of red blood cells fluxes of sodium before, during and after the cold pressor test, a method used also to evoke an adrenergic stimulation, in twenty hypertensive subjects, 14 males and 6 females, with an average age of 43.2 ± 5.7 years, with normal weight and without cardiovascular complications and metabolic diseases. The behaviour of the Na + total efflux (Na + TE), of the Na +/K + pump, of the Na +K + cotrasport (Na +/K + CT), of the Na +/Li + counter transport (Na +/Li + Cnt), of the Na + passive permeability (Na + PP), of the intracellular Na + (I Na +) and of the plasmatic noradrenaline (NE) was evaluated basally, at the third minute during cold pressor test (CPT) and 20 minutes after the end of the test. The test, wich the same method, was repeated after a 30 day treatment with propranolol at the dose of 240 mg/day in three daily administrations. The β-blockade caused, besides the reduction of both the systolic and diastolic pressure values, a significant increase in the Na +K + CT (from 248 ± 41 to 314 ± 71 mmol/l/cells/h, p <0.001) and a decrease in the Na + PP (from 0.039 ± 0.004 to 0.023 ± 0.007 hr 1, p <0.00001), probabily directed towards the reduction of the accumulation of intracellular Na +, that could compete, among the other mechanisms, with the anti-hypertensive action of the beta-blockers. The CPT caused, before the β-blockade, a significant depression of the Na +/K + pump (from 2057 ± 149 to 1610 ± 101 mmol/l/cells/h, p <0.00001) and of the Na + TE (from 2640 ± 397 to 2032 ± 179 mmol/l/cells/h, p <0.00001) inversely correlated to the levels of NE (r = - 0.60, p <0.003), with a consequent increase in I Na + (from 6.2 ± 0.6 to 7.5 ± 1.5 mmol/l/cells, p <0.001), showing how the adrenergic activation in hypertensive subjects is able to interfere with the systems of transmembrane transport with an inhibitory attitude, that is expressed by an increase in the levels of I Na +. The β-blockade was able to outweigh the depression of the Na +/K + pump (from 1843 ± 584 to 1728 ± 640 mmol/l/cells/h, p: ns) and the reduction of the Na + TE, preventing the accumulation of I Na + (from 6.3 ± 1.6 to 6.6 ± 1.3 mmol/l/cells, p: ns). Such data show an increased susceptibility of the Na + transport systems to the adrenergic stimuli in hypertensive subjects with a tendency to favor the accumulation of I Na + and that the β-blockade is able to antagonize the effects, with a maintenance of the intracellular levels of Na +.

Translated title of the contributionEffects of the β-blockade on the systems of red blood cells sodium transport: Evaluation during stimulus with cold pressor test in patients with essential hypertension
Original languageItalian
Pages (from-to)237-247
Number of pages11
JournalClinica Terapeutica
Volume148
Issue number5-6
Publication statusPublished - May 1997

ASJC Scopus subject areas

  • Medicine(all)

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    Saitta, A., Saitta, M. N., Bonaiuto, M., Castaldo, M., Sardo, A., Messina, A., Cinquegrani, M., Squadrito, G., Campo, G. M., & Squadrito, F. (1997). Effetti del β-blocco sui sistemi di transporto eritrocitario del Na +: Valutazione durante stimolo con cold pressor test in pazienti con ipertensione essenziale. Clinica Terapeutica, 148(5-6), 237-247.