Effects of the human cd38 glycoprotein on the early stages of the HIV-1 replication cycle

Andrea Savarino, Flavia Bottarel, Liliana Calosso, Maria Josè Feito, Thea Bensi, Manuela Bragardo, Josè Maria Rojo, Agostino Pugliese, Isabella Abbate, Maria R. Capobianchi, Ferdinando Dianzani, Fabio Malavasi, Umberto Dianzani

Research output: Contribution to journalArticle

Abstract

CD38 displays lateral association with the HIV-1 receptor CD4. This association is potentiated by the HIV-1 envelope glycoprotein gp120. The aim of this work was to evaluate the CD38 role in T cell susceptibility to HIV-1 infection. Using laboratory X4 HIV-1 strains and X4 and X4/R5 primary isolates, we found that CD38 expression was negatively correlated to cell susceptibility to infection, evaluated as percentage of infected cells, release of HIV p24 in the supernatants, and cytopathogenicity. This correlation was at first suggested by results obtained in a panel of human CD4+ T cell lines expressing different CD38 levels (MT-4, MT-2, C8166, CEMx174, Supt-1, and H9) and then demonstrated using CD38 transfectants of MT-4 cells (the line with the lowest CD38 expression). To address whether CD38 affected viral binding, we used mouse T cells that are non-permissive for productive infection. Gene transfection in mouse SR.D10.CD4-.F1 T cells produced four lines expressing human CD4 and/or CD38. Ability of CD4+CD38+ cells to bind HIV-1 or purified recombinant gp120 was significantly lower than that of CD4+CD38- cells. These data suggest that CD38 expression inhibits lymphocyte susceptibility to HIV infection, probably by inhibiting gp120/CD4-dependent viral binding to target cells. - Savarino, A., Bottarel, F., Calosso, L., Feito, M. J., Bensi, T., Bragardo, M., Rojo, J. M., Pugliese, A., Abbate, I., Capobianchi, M. R., Dianzani, F., Malavasi, F., and Dianzani, U. Effects of the human CD38 glycoprotein on the early stages of the HIV-1 replication cycle.

Original languageEnglish
Pages (from-to)2265-2276
Number of pages12
JournalFASEB Journal
Volume13
Issue number15
Publication statusPublished - 1999

Keywords

  • gp120/CD4-dependent virus binding
  • Susceptibility to HIV-1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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  • Cite this

    Savarino, A., Bottarel, F., Calosso, L., Feito, M. J., Bensi, T., Bragardo, M., Rojo, J. M., Pugliese, A., Abbate, I., Capobianchi, M. R., Dianzani, F., Malavasi, F., & Dianzani, U. (1999). Effects of the human cd38 glycoprotein on the early stages of the HIV-1 replication cycle. FASEB Journal, 13(15), 2265-2276.