Effects of the neuropeptide Y on estradiol and progesterone secretion by human granulosa cells in culture

Antonina Barreca, Barbara Valli, Arianna Cesarone, Marica Arvigo, Marcella Balasini, Gian Battista La Sala, Simona Garrone, Francesco Minuto, Giulio Giordano

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To investigate the possible effects of neuropeptide Y on steroid release by human granulosa cells in culture. Design: Prospective study. Setting: A university laboratory and the division of obstetrics and gynecology in a hospital. Patient(s): Sixteen normally ovulating women. Intervention(s): Ovulation induction for IVF-ET with an LH-releasing hormone analogue and gonadotropins. Main Outcome Measure(s): E2 and progesterone were assayed in the media conditioned by granulosa cells with the use of a double-antibody RIA. Result(s): Neuropeptide Y stimulates E2 production in a dose-dependent fashion. Preincubation for 3 hours with hCG led to a statistically significant increase in neuropeptide Y-induced E2 secretion. In contrast, whereas 3 hours of preincubation with 10-7 mol/L of neuropeptide Y did not elicit a statistically significant increase in hCG- induced E2 secretion, coincubation for 48 hours significantly increased hCG- stimulated secretion. Unlike E2, progesterone secretion did not undergo any statistically significant or dose-dependent variation after treatment with neuropeptide Y. Conclusion(s): Neuropeptide Y plays a role in human ovarian steroidogenesis directly at the level of the granulosa cells of the follicles in the early stage of luteinization. In this way, neuropeptide Y could play an important role in controlling the positive feedback effect exerted by the ovarian steroids on LH-releasing hormone and gonadotropins in humans.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalFertility and Sterility
Volume70
Issue number2
DOIs
Publication statusPublished - Aug 1998

Fingerprint

Granulosa Cells
Neuropeptide Y
Progesterone
Estradiol
Cell Culture Techniques
Gonadotropins
Gonadotropin-Releasing Hormone
Steroids
Luteinization
Ovulation Induction
Conditioned Culture Medium
Gynecology
Obstetrics
Outcome Assessment (Health Care)
Prospective Studies
Antibodies

Keywords

  • Estradiol
  • Human granulosa cells
  • Neuropeptide Y
  • Progesterone

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Effects of the neuropeptide Y on estradiol and progesterone secretion by human granulosa cells in culture. / Barreca, Antonina; Valli, Barbara; Cesarone, Arianna; Arvigo, Marica; Balasini, Marcella; La Sala, Gian Battista; Garrone, Simona; Minuto, Francesco; Giordano, Giulio.

In: Fertility and Sterility, Vol. 70, No. 2, 08.1998, p. 320-325.

Research output: Contribution to journalArticle

Barreca, A, Valli, B, Cesarone, A, Arvigo, M, Balasini, M, La Sala, GB, Garrone, S, Minuto, F & Giordano, G 1998, 'Effects of the neuropeptide Y on estradiol and progesterone secretion by human granulosa cells in culture', Fertility and Sterility, vol. 70, no. 2, pp. 320-325. https://doi.org/10.1016/S0015-0282(98)00133-2
Barreca, Antonina ; Valli, Barbara ; Cesarone, Arianna ; Arvigo, Marica ; Balasini, Marcella ; La Sala, Gian Battista ; Garrone, Simona ; Minuto, Francesco ; Giordano, Giulio. / Effects of the neuropeptide Y on estradiol and progesterone secretion by human granulosa cells in culture. In: Fertility and Sterility. 1998 ; Vol. 70, No. 2. pp. 320-325.
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