TY - JOUR
T1 - Effects of treatment in the levels of circulating cytokines and growth factors in cystic fibrosis and dialyzed patients by multi-analytical determination with a biochip array platform
AU - Tirelli, Amedea Silvia
AU - Colombo, Carla
AU - Torresani, Erminio
AU - Fortunato, Francesca
AU - Biffi, Arianna
AU - Cariani, Lisa
AU - Daccò, Valeria
AU - Carbone, Annalucia
AU - Edefonti, Alberto
AU - Paglialonga, Fabio
AU - Conese, Massimo
PY - 2013/6
Y1 - 2013/6
N2 - Chronic inflammatory diseases need non-invasive sensitive, reliable and predictive clinical biomarkers for diagnosis and monitoring therapy. Since inflammation is a complex phenomenon, simultaneous evaluation of different analytes in the same sample may help in defining this complexity and in developing specific anti-inflammatory intervention strategies. In this study, we used a biochip array system capable of measuring 12 cytokines and growth factors (IL-2, IL-4, IL-6, IL-8, IL-10, IL-1 α, IL-1 β, IFN-γ, TNF-α, MCP-1, VEGF, and EGF) in three groups: 97 control subjects; 24 cystic fibrosis (CF) patients before and during the antibiotic treatment (6 and 15. days) for acute pulmonary exacerbation as well as 15. days after the withdrawal of therapy; 22 children and young adults on chronic hemodialysis (HD) at the beginning and at the end of a standard HD session. CF patients in acute exacerbation displayed higher IL-2, IL-6, VEGF and MCP-1 levels than the control subjects. IL-6 significantly decreased during therapy (. P
AB - Chronic inflammatory diseases need non-invasive sensitive, reliable and predictive clinical biomarkers for diagnosis and monitoring therapy. Since inflammation is a complex phenomenon, simultaneous evaluation of different analytes in the same sample may help in defining this complexity and in developing specific anti-inflammatory intervention strategies. In this study, we used a biochip array system capable of measuring 12 cytokines and growth factors (IL-2, IL-4, IL-6, IL-8, IL-10, IL-1 α, IL-1 β, IFN-γ, TNF-α, MCP-1, VEGF, and EGF) in three groups: 97 control subjects; 24 cystic fibrosis (CF) patients before and during the antibiotic treatment (6 and 15. days) for acute pulmonary exacerbation as well as 15. days after the withdrawal of therapy; 22 children and young adults on chronic hemodialysis (HD) at the beginning and at the end of a standard HD session. CF patients in acute exacerbation displayed higher IL-2, IL-6, VEGF and MCP-1 levels than the control subjects. IL-6 significantly decreased during therapy (. P
KW - Biochip array technology
KW - Chronic renal disease
KW - Cystic fibrosis
KW - Cytokines
KW - Growth factors
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UR - http://www.scopus.com/inward/citedby.url?scp=84877796297&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2013.03.029
DO - 10.1016/j.cyto.2013.03.029
M3 - Article
C2 - 23608195
AN - SCOPUS:84877796297
VL - 62
SP - 413
EP - 420
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 3
ER -