Effects of triiodothyronine (T3) on differentiation of rat cortical neurons in primary cultures

A. Cestelli, D. Castiglia, C. Di Liegro, L. Bonfanti, I. Di Liegro

Research output: Contribution to journalArticlepeer-review

Abstract

Some of the events which characterize neuronal terminal differentiation have been studied in rat cortical neurons cultured in a selective synthetic medium for a period which corresponds to terminal brain maturation in vivo. In particular, we have studied the effect of T3 on the synthesis of nuclear proteins and the expression of the mRNAs which encode different variants of T3 nuclear receptors (c erb A proteins). We have shown that: a) T3 stimulates the turnover of nuclear proteins, with a more evident effect on the non-histone component; b) for the whole lifespan of cultures the predominant form of c erb Aα mRNA is the α2 variant (which encodes a protein unable to bind T3); whatever the function of α2 protein this finding suggests that its predominance on α1 is settled very early during mammalian brain maturation.

Original languageEnglish
Pages (from-to)168-171
Number of pages4
JournalCytotechnology
Volume5
Issue number1 Supplement
DOIs
Publication statusPublished - Feb 1991

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology

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