Effects of trypsin on the in situ identification of epidermal cell membrane antigens

G. Zambruno, V. Gielen, D. Schmitt, C. Dezutter-Dambuyant, J. Thivolet

Research output: Contribution to journalArticle

Abstract

In this work the role of trypsin in revealing epidermal cell surface antigens, with the use of immunological markers, was investigated. Two monoclonal antibodies (MCA) were used, the first: D47, belongs to the first cluster of differentiation and recognizes a membrane antigen of human thymocytes; the second HLA-ABC-m3, is an anti-HLA-B27 MCA. Preliminary treatment with various concentrations of trypsin was performed on frozen skin sections and followed by indirect immunofluorescence. D47 reacted with epidermal dentritic cells only after trypsin pretreatment of skin sections. In addition a mild preliminary trypsinization was shown to increase in situ immunoreactivity of MCA HLA-ABC-m3 with epidermal cells. Best results were obtained when trypsin concentrations ranging from 2.5 to 5 μg/gl were applied for 10 min at 37°C. Preliminary trypsinization may be of interest for a better exposure of some surface antigens to immunohistochemical markers.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalJournal of Cutaneous Pathology
Volume14
Issue number6
Publication statusPublished - 1987

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Trypsin
Cell Membrane
Antigens
Monoclonal Antibodies
Surface Antigens
HLA-B27 Antigen
Skin
Frozen Sections
Thymocytes
Indirect Fluorescent Antibody Technique
Membranes
Therapeutics

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

Cite this

Zambruno, G., Gielen, V., Schmitt, D., Dezutter-Dambuyant, C., & Thivolet, J. (1987). Effects of trypsin on the in situ identification of epidermal cell membrane antigens. Journal of Cutaneous Pathology, 14(6), 331-336.

Effects of trypsin on the in situ identification of epidermal cell membrane antigens. / Zambruno, G.; Gielen, V.; Schmitt, D.; Dezutter-Dambuyant, C.; Thivolet, J.

In: Journal of Cutaneous Pathology, Vol. 14, No. 6, 1987, p. 331-336.

Research output: Contribution to journalArticle

Zambruno, G, Gielen, V, Schmitt, D, Dezutter-Dambuyant, C & Thivolet, J 1987, 'Effects of trypsin on the in situ identification of epidermal cell membrane antigens', Journal of Cutaneous Pathology, vol. 14, no. 6, pp. 331-336.
Zambruno, G. ; Gielen, V. ; Schmitt, D. ; Dezutter-Dambuyant, C. ; Thivolet, J. / Effects of trypsin on the in situ identification of epidermal cell membrane antigens. In: Journal of Cutaneous Pathology. 1987 ; Vol. 14, No. 6. pp. 331-336.
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