Effects of various modes of mechanical ventilation in normal rats

Matteo Pecchiari, Ario Monaco, Antonia Koutsoukou, Patrizia Della Valle, Guendalina Gentile, Edgardo D'Angelo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Recent studies in healthy mice and rats have reported that positive pressure ventilation delivered with physiological tidal volumes at normal end-expiratory volume worsens lung mechanics and induces cytokine release, thus suggesting that detrimental effects are due to positive pressure ventilation per se. The aim of this study in healthy animals is to assess whether these adverse outcomes depend on the mode of mechanical ventilation. Methods: Rats were subjected to 4 h of spontaneous, positive pressure, and whole-body or thorax-only negative pressure ventilation (N = 8 per group). In all instances the ventilatory pattern was that of spontaneous breathing. Lung mechanics, cytokines concentration in serum and broncho-alveolar lavage fluid, lung wet-to-dry ratio, and histology were assessed. Values from eight animals euthanized shortly after anesthesia served as control. Results: No evidence of mechanical ventilation-dependent lung injury was found in terms of lung mechanics, histology, or wet-to-dry ratio. Relative to control, cytokine levels and recruitment of polymorphonuclear leucocytes increased slightly, and to the same extent with spontaneous, positive pressure, and whole-body negative pressure ventilation. Thorax-only negative pressure ventilation caused marked chest and lung distortion, reversible increase of lung elastance, and higher polymorphonuclear leucocyte count and cytokine levels. Conclusion: Both positive and negative pressure ventilation performed with tidal volumes and timing of spontaneous, quiet breathing neither elicit an inflammatory response nor cause morpho-functional alterations in normal animals, thus supporting the notion of the presence of a critical volume threshold above which acute lung injury ensues. Distortion of lung parenchyma can induce an inflammatory response, even in the absence of volotrauma.

Original languageEnglish
Pages (from-to)943-950
Number of pages8
JournalAnesthesiology
Volume120
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Artificial Respiration
Lung
Positive-Pressure Respiration
Pressure
Mechanics
Cytokines
Ventilation
Thorax
Tidal Volume
Histology
Respiration
Neutrophils
Acute Lung Injury
Bronchoalveolar Lavage Fluid
Lung Injury
Pulmonary Edema
Leukocyte Count
Anesthesia
Serum

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Pecchiari, M., Monaco, A., Koutsoukou, A., Valle, P. D., Gentile, G., & D'Angelo, E. (2014). Effects of various modes of mechanical ventilation in normal rats. Anesthesiology, 120(4), 943-950. https://doi.org/10.1097/ALN.0000000000000075

Effects of various modes of mechanical ventilation in normal rats. / Pecchiari, Matteo; Monaco, Ario; Koutsoukou, Antonia; Valle, Patrizia Della; Gentile, Guendalina; D'Angelo, Edgardo.

In: Anesthesiology, Vol. 120, No. 4, 2014, p. 943-950.

Research output: Contribution to journalArticle

Pecchiari, M, Monaco, A, Koutsoukou, A, Valle, PD, Gentile, G & D'Angelo, E 2014, 'Effects of various modes of mechanical ventilation in normal rats', Anesthesiology, vol. 120, no. 4, pp. 943-950. https://doi.org/10.1097/ALN.0000000000000075
Pecchiari, Matteo ; Monaco, Ario ; Koutsoukou, Antonia ; Valle, Patrizia Della ; Gentile, Guendalina ; D'Angelo, Edgardo. / Effects of various modes of mechanical ventilation in normal rats. In: Anesthesiology. 2014 ; Vol. 120, No. 4. pp. 943-950.
@article{c179f511b0754c319e1f9ec50bbc628c,
title = "Effects of various modes of mechanical ventilation in normal rats",
abstract = "Background: Recent studies in healthy mice and rats have reported that positive pressure ventilation delivered with physiological tidal volumes at normal end-expiratory volume worsens lung mechanics and induces cytokine release, thus suggesting that detrimental effects are due to positive pressure ventilation per se. The aim of this study in healthy animals is to assess whether these adverse outcomes depend on the mode of mechanical ventilation. Methods: Rats were subjected to 4 h of spontaneous, positive pressure, and whole-body or thorax-only negative pressure ventilation (N = 8 per group). In all instances the ventilatory pattern was that of spontaneous breathing. Lung mechanics, cytokines concentration in serum and broncho-alveolar lavage fluid, lung wet-to-dry ratio, and histology were assessed. Values from eight animals euthanized shortly after anesthesia served as control. Results: No evidence of mechanical ventilation-dependent lung injury was found in terms of lung mechanics, histology, or wet-to-dry ratio. Relative to control, cytokine levels and recruitment of polymorphonuclear leucocytes increased slightly, and to the same extent with spontaneous, positive pressure, and whole-body negative pressure ventilation. Thorax-only negative pressure ventilation caused marked chest and lung distortion, reversible increase of lung elastance, and higher polymorphonuclear leucocyte count and cytokine levels. Conclusion: Both positive and negative pressure ventilation performed with tidal volumes and timing of spontaneous, quiet breathing neither elicit an inflammatory response nor cause morpho-functional alterations in normal animals, thus supporting the notion of the presence of a critical volume threshold above which acute lung injury ensues. Distortion of lung parenchyma can induce an inflammatory response, even in the absence of volotrauma.",
author = "Matteo Pecchiari and Ario Monaco and Antonia Koutsoukou and Valle, {Patrizia Della} and Guendalina Gentile and Edgardo D'Angelo",
year = "2014",
doi = "10.1097/ALN.0000000000000075",
language = "English",
volume = "120",
pages = "943--950",
journal = "Anesthesiology",
issn = "0003-3022",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Effects of various modes of mechanical ventilation in normal rats

AU - Pecchiari, Matteo

AU - Monaco, Ario

AU - Koutsoukou, Antonia

AU - Valle, Patrizia Della

AU - Gentile, Guendalina

AU - D'Angelo, Edgardo

PY - 2014

Y1 - 2014

N2 - Background: Recent studies in healthy mice and rats have reported that positive pressure ventilation delivered with physiological tidal volumes at normal end-expiratory volume worsens lung mechanics and induces cytokine release, thus suggesting that detrimental effects are due to positive pressure ventilation per se. The aim of this study in healthy animals is to assess whether these adverse outcomes depend on the mode of mechanical ventilation. Methods: Rats were subjected to 4 h of spontaneous, positive pressure, and whole-body or thorax-only negative pressure ventilation (N = 8 per group). In all instances the ventilatory pattern was that of spontaneous breathing. Lung mechanics, cytokines concentration in serum and broncho-alveolar lavage fluid, lung wet-to-dry ratio, and histology were assessed. Values from eight animals euthanized shortly after anesthesia served as control. Results: No evidence of mechanical ventilation-dependent lung injury was found in terms of lung mechanics, histology, or wet-to-dry ratio. Relative to control, cytokine levels and recruitment of polymorphonuclear leucocytes increased slightly, and to the same extent with spontaneous, positive pressure, and whole-body negative pressure ventilation. Thorax-only negative pressure ventilation caused marked chest and lung distortion, reversible increase of lung elastance, and higher polymorphonuclear leucocyte count and cytokine levels. Conclusion: Both positive and negative pressure ventilation performed with tidal volumes and timing of spontaneous, quiet breathing neither elicit an inflammatory response nor cause morpho-functional alterations in normal animals, thus supporting the notion of the presence of a critical volume threshold above which acute lung injury ensues. Distortion of lung parenchyma can induce an inflammatory response, even in the absence of volotrauma.

AB - Background: Recent studies in healthy mice and rats have reported that positive pressure ventilation delivered with physiological tidal volumes at normal end-expiratory volume worsens lung mechanics and induces cytokine release, thus suggesting that detrimental effects are due to positive pressure ventilation per se. The aim of this study in healthy animals is to assess whether these adverse outcomes depend on the mode of mechanical ventilation. Methods: Rats were subjected to 4 h of spontaneous, positive pressure, and whole-body or thorax-only negative pressure ventilation (N = 8 per group). In all instances the ventilatory pattern was that of spontaneous breathing. Lung mechanics, cytokines concentration in serum and broncho-alveolar lavage fluid, lung wet-to-dry ratio, and histology were assessed. Values from eight animals euthanized shortly after anesthesia served as control. Results: No evidence of mechanical ventilation-dependent lung injury was found in terms of lung mechanics, histology, or wet-to-dry ratio. Relative to control, cytokine levels and recruitment of polymorphonuclear leucocytes increased slightly, and to the same extent with spontaneous, positive pressure, and whole-body negative pressure ventilation. Thorax-only negative pressure ventilation caused marked chest and lung distortion, reversible increase of lung elastance, and higher polymorphonuclear leucocyte count and cytokine levels. Conclusion: Both positive and negative pressure ventilation performed with tidal volumes and timing of spontaneous, quiet breathing neither elicit an inflammatory response nor cause morpho-functional alterations in normal animals, thus supporting the notion of the presence of a critical volume threshold above which acute lung injury ensues. Distortion of lung parenchyma can induce an inflammatory response, even in the absence of volotrauma.

UR - http://www.scopus.com/inward/record.url?scp=84901706206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901706206&partnerID=8YFLogxK

U2 - 10.1097/ALN.0000000000000075

DO - 10.1097/ALN.0000000000000075

M3 - Article

VL - 120

SP - 943

EP - 950

JO - Anesthesiology

JF - Anesthesiology

SN - 0003-3022

IS - 4

ER -