Effects of verbascoside, biotechnologically purified by Syringa vulgaris plant cell cultures, in a rodent model of periodontitis

Rosanna D I Paola, Giacomo Oteri, Emanuela Mazzon, Concetta Crisafulli, Maria Galuppo, Roberto D A L Toso, Giovanna Pressi, Giancarlo Cordasco, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

Abstract

Objectives Verbascoside has previously been characterized as an effective scavenger of active free radicals and an inhibitor of lipid peroxidation. In the present study, we have investigated the effects of verbascoside from Syringa vulgaris in a rat model of ligature-induced periodontitis. Methods Male Sprague-Dawley rats were lightly anaesthetized with pentobarbitone (35 mg/kg). Sterile, 2-0 black braided silk thread was placed around the cervix of the lower left first molar and knotted medially. Animals received vebascoside 2 mg/kg orally, daily for 8 days. Key findings On the eighth day after placement of the ligature, we evaluated several markers of inflammation: (i) myeloperoxidase activity, (ii) thiobarbituric acid-reactant substance measurements, (iii) NF-κB expression, (iv) iNOS expression, (v) the nitration of tyrosine residues, (vi) activation of the nuclear enzyme poly(ADP-ribose) polymerase, (vii) Bax and Bcl-2 expression and (viii) a degree of gingivomucosal tissue injury. Oral administration of verbascoside (2 mg/kg daily for 8 days) significantly decreased all of the parameters of inflammation as described above. Conclusions These results demonstrate that verbascoside exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage associated with ligature-induced periodontitis.

Original languageEnglish
Pages (from-to)707-717
Number of pages11
JournalJournal of Pharmacy and Pharmacology
Volume63
Issue number5
DOIs
Publication statusPublished - May 2011

Keywords

  • alveolar bone loss
  • apoptosis
  • periodontal diseases
  • reactive oxygen species
  • verbascoside

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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